Isolation of primary mice splenocytes for in vitro research

Fawzi O. Ebrahim; Abdul M. Abolayha; Adam A. Elzagheid; Fadella Abosrer

doi:10.5455/ajvs.82519

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AJVS. 2020; 64(2): 13-20

doi: 10.5455/ajvs.82519

Isolation of primary mice splenocytes for in vitro research

Fawzi O. Ebrahim, Abdul M. Abolayha, Adam A. Elzagheid, Fadella Abosrer.

Abstract
Abstract
Animal cell culture is of great importance in research. We isolated mouse splenocytes from Swiss albino mice by using a mechanical method and cultured them in medium with fetal bovine serum, 1% penicillin-streptomycin and 1% amphotericin as an antibiotic and an antifungal, but without mitogen. The cells were confluent after 72 h, and their viability, measured by trypan blue assay, was 95%. The splenocytes were passaged four times and no fungal or bacterial contamination was observed during carrying out the experiment.

Key words: Key words: mouse spleen, splenocytes, trypan blue

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How to Cite this Article

Pubmed Style

Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519

Web Style

Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. https://www.alexjvs.com/?mno=82519 [Access: May 04, 2025]. doi:10.5455/ajvs.82519

AMA (American Medical Association) Style

Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519

Vancouver/ICMJE Style

Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. (2020), [cited May 04, 2025]; 64(2): 13-20. doi:10.5455/ajvs.82519

Harvard Style

Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. AJVS, 64 (2), 13-20. doi:10.5455/ajvs.82519

Turabian Style

Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. 2020. Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519

Chicago Style

Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. "Isolation of primary mice splenocytes for in vitro research." Alexandria Journal of Veterinary Sciences 64 (2020), 13-20. doi:10.5455/ajvs.82519

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519

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