E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples

Alaa Eldin H. Mustapha, Maha M. Mansour.


Abstract
A total of 260 samples of raw milk were gathered from a variety of farms, street vendors, residences, and dairy stores in the governorate of EL-Menoufia. 220 (84.6%) of the 260 samples had Enterobacteriaceae. At species level, Klebsiella and E. coli were most predominant at 40.4% and 32.6% respectively, followed by 21.2% Citrobacter freundii and 7.3% Proteus while Salmonella showed the lowest 1.9%. Using SYBER green real-time PCR, thirty uncultured samples were subjected to rpoB genotypic identification for viable Enterobacteriaceae detection, and all thirty (100%) of the samples showed the gene. By using SYBER green real-time PCR after PMA treatment, 10 (33.3%) of these 30 culture-negative samples showed the presence of VBNC enterobacterial isolates.

Key words: Propidium Monoazide PCR, real-time PCR, and viable but non-cultivable Enterobacteriaceae


 
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Pubmed Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. 2024; 81(0): 38-50. doi:10.5455/ajvs.196375

Web Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. https://www.alexjvs.com/?mno=196375 [Access: December 02, 2024]. doi:10.5455/ajvs.196375

AMA (American Medical Association) Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. 2024; 81(0): 38-50. doi:10.5455/ajvs.196375


Vancouver/ICMJE Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. (2024), [cited December 02, 2024]; 81(0): 38-50. doi:10.5455/ajvs.196375


Harvard Style

Mustapha, A. E. H. & Mansour, . M. M. (2024) Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS, 81 (0), 38-50. doi:10.5455/ajvs.196375


Turabian Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. 2024. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. Alexandria Journal of Veterinary Sciences, 81 (0), 38-50. doi:10.5455/ajvs.196375


Chicago Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. "Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples." Alexandria Journal of Veterinary Sciences 81 (2024), 38-50. doi:10.5455/ajvs.196375


MLA (The Modern Language Association) Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. "Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples." Alexandria Journal of Veterinary Sciences 81.0 (2024), 38-50. Print. doi:10.5455/ajvs.196375


APA (American Psychological Association) Style

Mustapha, A. E. H. & Mansour, . M. M. (2024) Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. Alexandria Journal of Veterinary Sciences, 81 (0), 38-50. doi:10.5455/ajvs.196375




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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