E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 81
81Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Review Article

Online Publishing Date:
21 / 04 / 2024

 


Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review

Alaa Eldin H. Mustapha, Maha M. Mansour.


Abstract
Microorganisms in their natural habitats are subjected to a broad range of stressors. In order to survive and retain their vitality under those demanding conditions, they enter a state known as viable but nonculturable (VBNC). Traditional laboratory techniques cannot identify microbes in the VBNC state, but they can be brought back to life in the right circumstances. As a result, VBNC pathogens seriously jeopardize public health and food safety. More than 100 microbe species have been found to have entered the VBNC state to date, owing to a variety of chemical and physical processes. Since the VBNC syndrome was first discovered forty years ago, new methods for inducing, detecting, resuscitating, and understanding its molecular pathways have been created. To determine their effect on public health, live bacterial foodborne pathogens must be found and measured. Due to the existence of viable but nonculturable cells (VBNC), which can retain metabolic and pathogenic activity, traditional growth-based approaches have the potential to significantly underestimate the true numbers. The similar issue arises when using different culture-independent molecular techniques, such PCR, which can detect DNA from dead cells in addition to VBNC bacteria. Viability dyes and PCR have been used to selectively identify and quantify viable foodborne microorganisms, overcoming this drawback.

Key words: Viable but non culturable (VBNC), real-time PCR, Propidium Monoazide PCR


 
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Pubmed Style

Mustapha AEH, Mansour MM. Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. AJVS. Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372

Web Style

Mustapha AEH, Mansour MM. Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. https://www.alexjvs.com/?mno=196372 [Access: May 14, 2024]. doi:10.5455/ajvs.196372

AMA (American Medical Association) Style

Mustapha AEH, Mansour MM. Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. AJVS. Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372


Vancouver/ICMJE Style

Mustapha AEH, Mansour MM. Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. AJVS, [cited May 14, 2024]; Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372


Harvard Style

Mustapha, A. E. H. & Mansour, . M. M. (2024) Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. AJVS, Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372


Turabian Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. 2024. Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. Alexandria Journal of Veterinary Sciences, Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372


Chicago Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. "Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review." Alexandria Journal of Veterinary Sciences Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372


MLA (The Modern Language Association) Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. "Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review." Alexandria Journal of Veterinary Sciences Online First: 21 Apr, 2024. Web. 14 May 2024 doi:10.5455/ajvs.196372


APA (American Psychological Association) Style

Mustapha, A. E. H. & Mansour, . M. M. (2024) Detection Of Viable but Non Culturable (VBNC) Bacteria Using of Propidium Monoazide PCR: A Review. Alexandria Journal of Veterinary Sciences, Online First: 21 Apr, 2024. doi:10.5455/ajvs.196372




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