E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes

Aml A. Bakheet, Dena E. Torra.

Abstract
This work was designed to investigate incidence of P. aeruginosa in dead in shell chicken embryos and molecular detection of virulence genes and study their pathogenicity effect on embryonated chicken eggs and chicks. Out of 100 pooled samples were collected from liver, heart and yolk sac of dead in shell chicken embryos, a total of 24 (24%) P. aeruginosa isolates were isolated.On carrying PCR test for 24 P. aeruginosa isolates there were high distribution of (opr L) and ( lasI ) genes with 100% while distribution of other virulence genes was as follows: toxA (83.3%), lasR (75%), Rhl1 (70.8%). Pathogenicity of P. aeruginosa on embryonated chicken eggs was studied either by inoculation of P. aeruginosa strain via yolk sac route in a dose of 0.1 ml suspension 14x10⁷. Which gave 100% embryonic mortalities after 3 days or by dipping group of 17 days old embryonated chicken eggs in a dose of 6.1X 109 /ml which gave 90% embryonic mortality. Experimentaly infected chicks showed 70% mortality. Histopathological changes in dead embryos from infected embryonated eggs and sacrificed, dead infected chicks revealed lymphocytic hepatitis with presence of gram negative bacteria. Lymphocytic nephritis with hemosiderosis found in renal parenchyma. Heart showed lymphocytic myocarditis. Lung showed alveolar emphysema, interstitial pneumonia and perivascular fibrosis. P. aeruginosa was re-isolated from internal organs of sacrificed hatched chicks and from liver and yolk sac of dead embryos.

Key words: P. aeruginosa, Chicken embryo, PCR, virulence genes, Histopathology


 
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How to Cite this Article
Pubmed Style

Bakheet AA, Torra DE. Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. AJVS. 2020; 65(1): 81-89. doi:10.5455/ajvs.101343


Web Style

Bakheet AA, Torra DE. Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. http://www.alexjvs.com/?mno=101343 [Access: November 27, 2020]. doi:10.5455/ajvs.101343


AMA (American Medical Association) Style

Bakheet AA, Torra DE. Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. AJVS. 2020; 65(1): 81-89. doi:10.5455/ajvs.101343



Vancouver/ICMJE Style

Bakheet AA, Torra DE. Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. AJVS. (2020), [cited November 27, 2020]; 65(1): 81-89. doi:10.5455/ajvs.101343



Harvard Style

Bakheet, A. A. & Torra, . D. E. (2020) Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. AJVS, 65 (1), 81-89. doi:10.5455/ajvs.101343



Turabian Style

Bakheet, Aml A., and Dena E. Torra. 2020. Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. Alexandria Journal of Veterinary Sciences, 65 (1), 81-89. doi:10.5455/ajvs.101343



Chicago Style

Bakheet, Aml A., and Dena E. Torra. "Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes." Alexandria Journal of Veterinary Sciences 65 (2020), 81-89. doi:10.5455/ajvs.101343



MLA (The Modern Language Association) Style

Bakheet, Aml A., and Dena E. Torra. "Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes." Alexandria Journal of Veterinary Sciences 65.1 (2020), 81-89. Print. doi:10.5455/ajvs.101343



APA (American Psychological Association) Style

Bakheet, A. A. & Torra, . D. E. (2020) Detection of Pseudomonas aeruginosa in Dead Chicken Embryo with Reference to Pathological Changes and Virulence Genes. Alexandria Journal of Veterinary Sciences, 65 (1), 81-89. doi:10.5455/ajvs.101343





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