E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats

Rasha T. Emarha, Nabil M. Taha, Abed Elwhab A. Mandour, Mohamed A. Lebda, Mohamed A. Rashed.

Abstract
This study was conducted to investigate the effect of moringa olifera and colchicine on thioacetamide induced liver fibrosis in rats. One hundred and fife rats were randomly divided into equal seven groups of fifteen animals each for 60 days as follows: The first group (control) was kept on basal diet and water, the second group hepatotoxicity was induced by i.p injection of TAA (150 mg/kg of body weight, i.p.), for three times/week for two months and the third group was given colchicine (COL) orally with stomach tube (50 μ/kg day ) the fourth group was given MOEL( moringa olifera ethanolic leaves extract) also by stomach tube ( 400 mg/kg) ,the fifth group was given TAA(150 mg/kg) + COL(50 μ/kg day ), the six group was given TAA(150 mg/kg) +MOEL(400mg/kg), the seven group was given (TAA(150 mg/kg) + COL(50 μ/kg day )+ MOEL 400mg/kg) for 3times/week for 2 months. We studied liver fibrosis from three point of view biochemical effect, molecular effect and histopathological effect. Blood samples were collected and serum was separated for the determination of the following biochemical tests as liver enzymatic function ALT (Alanin aminotransferase),AST (Aspartateaminotransferase),GGT, ALP (Alkaline phosphatase ) , total protein ,albumin. The total antioxidant capacity (TAC), reduced glutathione (GSH) and malondialdehyde (MDA) in liver tissue homogenates, molecular view point as measuring BAX (bacl2associated X, Apoptosis Regulator) Bcla2 (B-celleymphoma 2) for detection apoptosis occurred then histopathological effect of liver fibrosis on liver tissue.
The induction of TAA revealed a significant increase in liver enzymes, bilirubin, MDA, BAX, and a significant decrease in TP, Alb ,Bacl2 by p<0.05 as compared to control group while administration of COL and/ or MOEL leading to significant improvement in all liver function tests and improvement apoptosis by increasing significantly anti-apoptotic gene expiration (Bacl2) and decreasing pro-apoptotic gene expiration (BAX).also leading to regression accumulate to collagen fiber in liver cells. CONCLUTION: either colchicine or moringa olifera can be used as an adjuvant drug in treatment of liver fibrosis.

Key words: liver fibrosis, moringa olifera, colchicine, apoptosis


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Rasha T. Emarha
Articles by Nabil M. Taha
Articles by Abed Elwhab A. Mandour
Articles by Mohamed A. Lebda
Articles by Mohamed A. Rashed
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Emarha RT, Taha NM, Mandour AEA, Lebda MA, Rashed MA. Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. AJVS. 2021; 69(2): 7-15. doi:10.5455/ajvs.1886


Web Style

Emarha RT, Taha NM, Mandour AEA, Lebda MA, Rashed MA. Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. http://www.alexjvs.com/?mno=1886 [Access: June 29, 2021]. doi:10.5455/ajvs.1886


AMA (American Medical Association) Style

Emarha RT, Taha NM, Mandour AEA, Lebda MA, Rashed MA. Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. AJVS. 2021; 69(2): 7-15. doi:10.5455/ajvs.1886



Vancouver/ICMJE Style

Emarha RT, Taha NM, Mandour AEA, Lebda MA, Rashed MA. Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. AJVS. (2021), [cited June 29, 2021]; 69(2): 7-15. doi:10.5455/ajvs.1886



Harvard Style

Emarha, R. T., Taha, . N. M., Mandour, . A. E. A., Lebda, . M. A. & Rashed, . M. A. (2021) Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. AJVS, 69 (2), 7-15. doi:10.5455/ajvs.1886



Turabian Style

Emarha, Rasha T., Nabil M. Taha, Abed Elwhab A. Mandour, Mohamed A. Lebda, and Mohamed A. Rashed. 2021. Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. Alexandria Journal of Veterinary Sciences, 69 (2), 7-15. doi:10.5455/ajvs.1886



Chicago Style

Emarha, Rasha T., Nabil M. Taha, Abed Elwhab A. Mandour, Mohamed A. Lebda, and Mohamed A. Rashed. "Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats." Alexandria Journal of Veterinary Sciences 69 (2021), 7-15. doi:10.5455/ajvs.1886



MLA (The Modern Language Association) Style

Emarha, Rasha T., Nabil M. Taha, Abed Elwhab A. Mandour, Mohamed A. Lebda, and Mohamed A. Rashed. "Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats." Alexandria Journal of Veterinary Sciences 69.2 (2021), 7-15. Print. doi:10.5455/ajvs.1886



APA (American Psychological Association) Style

Emarha, R. T., Taha, . N. M., Mandour, . A. E. A., Lebda, . M. A. & Rashed, . M. A. (2021) Biochemical and Molecular Effects of Colchicine and Moringa Olifera on Thioacetamide Induced Liver Fibrosis in Rats. Alexandria Journal of Veterinary Sciences, 69 (2), 7-15. doi:10.5455/ajvs.1886





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]