E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) Open Access


Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels

Emad N. Hegazy, Abd El-Kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, Amir H. Abd El-Rahman.

Abstract
This study was designed to evaluate PCR technique as a diagnostic tool for blood parasites infection in equine and camels in relation to conventional microscopic examination of stained blood smear. 150 equine stained blood smears were examined microscopically, 15 of them were suspected to be infested with equine piroplasmosis. 10 samples (including one positive by blood film) were examined by conventional PCR technique, 7 samples were positive. 135 camels stained blood smears were examined microscopically, 2 of them were infested with trypanosoma, and 10, 5 and 15 samples were suspected to be infested with theileria spp., babesia spp. and anaplasma spp., respectively. 10 samples (including 2 positive for trypanosoma), 10 negative for theileria, 10 negative for babesia and 10 negative for anaplasma by blood film were examined separately by conventional PCR technique, 9, 1, 1 and 5 samples were positive, respectively. The results concluded that PCR assays are more sensitive than blood film examination.

Key words: PCR, blood smear, equine, camels, piroplasmois, Trypansoma, Theileria, Babesia, Anaplasma


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Emad N. Hegazy
Articles by Abd El-Kareem A. Mahmoud
Articles by Adel M. Khadr
Articles by Tharwat M. Elshemey
Articles by Amir H. Abd El-Rahman
on Google
on Google Scholar
Article Statistics
 Viewed: 78
Downloaded: 36
Cited: 0

How to Cite this Article
Pubmed Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-Rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. 2017; 55(1): 217-224. doi:10.5455/ajvs.261935


Web Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-Rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. http://www.alexjvs.com//?mno=261935 [Access: December 18, 2017]. doi:10.5455/ajvs.261935


AMA (American Medical Association) Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-Rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. 2017; 55(1): 217-224. doi:10.5455/ajvs.261935



Vancouver/ICMJE Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-Rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. (2017), [cited December 18, 2017]; 55(1): 217-224. doi:10.5455/ajvs.261935



Harvard Style

Hegazy, E. N., Mahmoud, A. E. A., Khadr, A. M., Elshemey, T. M. & El-Rahman, A. H. A. (2017) Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS, 55 (1), 217-224. doi:10.5455/ajvs.261935



Turabian Style

Hegazy, Emad N., Abd El-Kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-Rahman. 2017. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. Alexandria Journal of Veterinary Sciences, 55 (1), 217-224. doi:10.5455/ajvs.261935



Chicago Style

Hegazy, Emad N., Abd El-Kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-Rahman. "Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels." Alexandria Journal of Veterinary Sciences 55 (2017), 217-224. doi:10.5455/ajvs.261935



MLA (The Modern Language Association) Style

Hegazy, Emad N., Abd El-Kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-Rahman. "Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels." Alexandria Journal of Veterinary Sciences 55.1 (2017), 217-224. Print. doi:10.5455/ajvs.261935



APA (American Psychological Association) Style

Hegazy, E. N., Mahmoud, A. E. A., Khadr, A. M., Elshemey, T. M. & El-Rahman, A. H. A. (2017) Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. Alexandria Journal of Veterinary Sciences, 55 (1), 217-224. doi:10.5455/ajvs.261935





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Surgical and Histological Evaluation of the Effectiveness of Propolis on Wound Healing
    Ramadan El Sayed Abd Elwahed, Mahmoud Hussein El Kammar, Islam Reda Edrees
    AJVS. 2013; 39(1): 52-63
    » Abstract & References

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Improving Production Traits for El-salam and Mandarah Chicken Strains by Crossing I- Estimation of Crossbreeding Effects for Growth Production Traits
    Ayman El-Sayed Taha, Fawzy Ali Abd El-Ghany
    AJVS. 2013; 39(1): 18-30
    » Abstract & References

  • Most Downloaded
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Aerobic Bacteria Associated with Omphalitis of Chicks [English]
    Khalil, S. A. and Einas, El-Shamy
    AJVS. 2012; 37(1): 69-77
    » Abstract

  • Surgical and Histological Evaluation of the Effectiveness of Propolis on Wound Healing
    Ramadan El Sayed Abd Elwahed, Mahmoud Hussein El Kammar, Islam Reda Edrees
    AJVS. 2013; 39(1): 52-63
    » Abstract & References

  • Using Double Dose of GnRH for Reducing Incidence of Cystic Ovaries in Cows
    Wael MB Noseir, Kamal K Metwally, Negm N Shaker
    AJVS. 2013; 39(1): 124-132
    » Abstract & References » doi: 10.5455/ajvs.40648

  • Most Cited Articles
  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 3 times [Click to see citing articles]

  • Enumeration and Characterization of Aeromonas spp. Isolated from Milk and Some Dairy Products in Sharkia Governorate, Egypt
    Nagwa I. ElBalat, Salah F. Abd-ElAal, Madeha A. Ayoub, Magdy S. Elsayed
    AJVS. 2014; 40(1): 52-64
    » Abstract & References » doi: 10.5455/ajvs.49073
    Cited : 2 times [Click to see citing articles]

  • Comparison of Lidocaine and Lidocaine–Neostigmine for Epidural Analgesia in Water Buffalo Calves (Bubalus Bubalis)
    Alaa Ghazy, Ayman Atiba, Mustafa Shukry, Tarek Kamal
    AJVS. 2015; 46(1): 177-181
    » Abstract & References » doi: 10.5455/ajvs.198171
    Cited : 2 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 2 times [Click to see citing articles]

  • Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons
    Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.
    AJVS. 2015; 46(1): 1-14
    » Abstract & References » doi: 10.5455/ajvs.186038
    Cited : 2 times [Click to see citing articles]

  • Enterobacteriaceae In Beef Products (Luncheon, Pasterma, Frankfurter and Minced meat) from Alexandria Retail Outlets
    Noha M. El-Gendy, Hossam A. Ibrahim, Nahla A. Al-Shabasy, and Ibrahim A. Samaha
    AJVS. 2014; 41(1): 80-86
    » Abstract & References » doi: 10.5455/ajvs.151171
    Cited : 2 times [Click to see citing articles]

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307
    Cited : 1 time [Click to see citing article]

  • Incidence of Yersinia Enterocolitica and Yersinia Pseudotuberculosis in Raw Milk Samples of Different Animal Species Using Conventional and Molecular Methods
    Samah F. Darwish, Hanaa A.E. Asfour and Hanaa A. Allam
    AJVS. 2015; 44(1): 174-185
    » Abstract & References » doi: 10.5455/ajvs.176360
    Cited : 1 time [Click to see citing article]

  • Prevalence of Some Pathogens in a Population of Zoo Animals
    Mostafa A. Mahmoud
    AJVS. 2015; 45(1): 139-145
    » Abstract & References » doi: 10.5455/ajvs.184003
    Cited : 1 time [Click to see citing article]