E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel.

Cited by (3)

Abstract
Lead is a highly prevalent heavy metal pollutant in human and animals which has various toxicological effects on the different organs including reproductive system on particular aspect the male testis. Lead (Pb) exposure enhances the generation of Reactive Oxygen Species (ROS) which negatively affect testis structure and functions. The present study aimed to investigate the effect of garlic supplement on daily basis and its prophylactic possibility against lead acetate induced testicular dysfunction. Thirty two adult male rats were divided to four groups, eight animals per each. All animals were maintained under standard laboratory conditions with free access to suitable diet and water supply. The 1st group was supplied with water and diet and kept without further treatment as a negative control. The 2nd group was treated by lead acetate( 50mg/L) daily in drinking water as a positive control. The 3rd group was administered commercial garlic powder preparation only (200 mg/kg b.w.) by stomach tube. The 4th group was co-treated with lead acetate in drinking water plus garlic in same doses by stomach tube. Then, all animals were scarified after 42 days for clean excision of epididymis and testes. Semen was collected and analyzed for count, motility and viability. Testicular tissues sections were prepared, stained and examined. Oxidative stress markers, SOD and MDA were determined in testicular tissue homogenate. Immunostaining was carried out on sections of testicular tissue for detection of proapoptotic markers caspase-3 and Bax expression. Finally, the level of expression of Bcl-2 and P53 genes was measured in testicular tissue samples as well. The data summary obtained from co treated group signified the protective effect of garlic against potential lead acetate induced undesired testicular apoptosis.

Key words: Lead toxicity, apoptosis, male rat, testis, garlic


 
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This Article Cited By the following articles

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Ameliorative effects of Spirulina maxima and Allium sativum on lead acetate-induced testicular injury in male albino rats with respect to caspase-3 gene expression
Acta Histochemica 2019; 121(2): 198.

2
 
Ameliorative effects of Spirulina maxima and Allium sativum on lead acetate-induced testicular injury in male albino rats with respect to caspase-3 gene expression
Acta Histochemica 2018; (): .

3
 
How to Cite this Article
Pubmed Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. AJVS. 2017; 54(1): 70-78. doi:10.5455/ajvs.268149


Web Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. http://www.alexjvs.com/?mno=268149 [Access: July 22, 2019]. doi:10.5455/ajvs.268149


AMA (American Medical Association) Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. AJVS. 2017; 54(1): 70-78. doi:10.5455/ajvs.268149



Vancouver/ICMJE Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. AJVS. (2017), [cited July 22, 2019]; 54(1): 70-78. doi:10.5455/ajvs.268149



Harvard Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel (2017) Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. AJVS, 54 (1), 70-78. doi:10.5455/ajvs.268149



Turabian Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. 2017. Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. Alexandria Journal of Veterinary Sciences, 54 (1), 70-78. doi:10.5455/ajvs.268149



Chicago Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. "Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats." Alexandria Journal of Veterinary Sciences 54 (2017), 70-78. doi:10.5455/ajvs.268149



MLA (The Modern Language Association) Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel. "Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats." Alexandria Journal of Veterinary Sciences 54.1 (2017), 70-78. Print. doi:10.5455/ajvs.268149



APA (American Psychological Association) Style

Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel (2017) Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats. Alexandria Journal of Veterinary Sciences, 54 (1), 70-78. doi:10.5455/ajvs.268149





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