E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil.

Abstract
Three commercial products of herbal extracts (Amphiflu-Ex«, Fyto-immune Plus« and Viu-Vet«) were tested for antiviral activity against two Egyptian highly pathogenic avian influenza subtype H5N1 isolates (isolated form backyard poultry) and upon sequencing and phylogenetic analysis, they belonged to clade 2.2.1.2., This antiviral assay for the products done by using chicken embryo fibroblast (CEF) cell culture through cytopathic effect (CPE) inhibition assay in two different layouts to elucidate post-exposure and pre-exposure antiviral activity of the products. The Amphiflu-Ex« at conc. 50 Ál/ml showed significant virucidal activity (100% inhibition) in post-exposure assay for both viral isolates which was further confirmed by quantitative real time RT-PCR method showed 100% reduction in virus titers for both isolates and further investigation done on The Amphiflu-Ex« on conc. 20 Ál/ml, the virus titers were decreased by 2.72-2.8 logs (Log 10 virus titers) and on conc 10 Ál/ml, the virus titers were decreased by 1-1.27 logs for both isolates, The maximum non-toxic concentration (MNTC) for the three products estimated by cytotoxicity assay was 50 Ál/ml and TCID50 used for both isolates were 106 copy/100 Ál.

Key words: Avian influenza virus subtype- H5N1, Antiviral activity, Herbal extracts, RT-PCR.


 
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Pubmed Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. 2018; 56(1): 145-152. doi:10.5455/ajvs.281430


Web Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. http://www.alexjvs.com/?mno=281430 [Access: December 12, 2018]. doi:10.5455/ajvs.281430


AMA (American Medical Association) Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. 2018; 56(1): 145-152. doi:10.5455/ajvs.281430



Vancouver/ICMJE Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. (2018), [cited December 12, 2018]; 56(1): 145-152. doi:10.5455/ajvs.281430



Harvard Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil (2018) In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS, 56 (1), 145-152. doi:10.5455/ajvs.281430



Turabian Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. 2018. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. Alexandria Journal of Veterinary Sciences, 56 (1), 145-152. doi:10.5455/ajvs.281430



Chicago Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. "In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus." Alexandria Journal of Veterinary Sciences 56 (2018), 145-152. doi:10.5455/ajvs.281430



MLA (The Modern Language Association) Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil. "In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus." Alexandria Journal of Veterinary Sciences 56.1 (2018), 145-152. Print. doi:10.5455/ajvs.281430



APA (American Psychological Association) Style

Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil (2018) In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. Alexandria Journal of Veterinary Sciences, 56 (1), 145-152. doi:10.5455/ajvs.281430





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