E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens

Nehal M. Nabil, Ahlam E. Yonis.

Abstract
This study was carried out to isolate Salmonella from different broiler farms in Delta, Egypt and to investigate their antimicrobial susceptibility pattern with a molecular detection of genes responsible for biofilm formation and resistance to disinfectants. Five Egyptian Governorates in Delta (Dakahila, Damietta, Gharbia, Kafr El Sheik and Sharkia) during the year 2018 were included in this study. A total of 100 diseased farms were subjected for clinical followed by laboratory diagnosis. Clinical diagnosis was made on the basis of clinical history, signs and postmortem findings of the affected broiler chickens. Internal organs (liver, cecum, spleen and heart) and swabs form water pipes, waterers and feeders were collected. Salmonella was isolated with an incidence rate of (17%). The incidences of Salmonella isolation from the collected samples in Dakahila, Damietta, Gharbia, Kafr El Sheik and Sharkia Governorates were (5/29) (17.24%), (4/23) (17.39%), (3/20) (15%), (2/13) (15.38%) and (3/15) (20%) respectively. The isolated Salmonellae were identified serologically and six serotypes were reported (S. Typhimurium, S. Kentucky, S. Inganda, S. Infantis, S. Newport and S. Enteritidis). Antimicrobial sensitivity testing revealed that ampicillin sulbactam, tetracycline and streptomycin showed higher resistance with percentages (94.1%), (94.1%) and (70.6%) respectively. Using conventional PCR technique; AdrA, gcpA and csgD genes that responsible for biofilm formation were reported in all Salmonella isolates. qacED1 gene was detected with percentages of (100%) but qacA/B and qacC/D genes were negative.

Key words: Keywords: Salmonella, biofilm, disinfectant resistance, broiler


 
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How to Cite this Article
Pubmed Style

Nabil NM, Yonis AE. Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. AJVS. 2019; 62(2): 26-36. doi:10.5455/ajvs.57274


Web Style

Nabil NM, Yonis AE. Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. http://www.alexjvs.com/?mno=57274 [Access: February 09, 2020]. doi:10.5455/ajvs.57274


AMA (American Medical Association) Style

Nabil NM, Yonis AE. Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. AJVS. 2019; 62(2): 26-36. doi:10.5455/ajvs.57274



Vancouver/ICMJE Style

Nabil NM, Yonis AE. Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. AJVS. (2019), [cited February 09, 2020]; 62(2): 26-36. doi:10.5455/ajvs.57274



Harvard Style

Nabil, N. M. & Yonis, . A. E. (2019) Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. AJVS, 62 (2), 26-36. doi:10.5455/ajvs.57274



Turabian Style

Nabil, Nehal M., and Ahlam E. Yonis. 2019. Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. Alexandria Journal of Veterinary Sciences, 62 (2), 26-36. doi:10.5455/ajvs.57274



Chicago Style

Nabil, Nehal M., and Ahlam E. Yonis. "Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens." Alexandria Journal of Veterinary Sciences 62 (2019), 26-36. doi:10.5455/ajvs.57274



MLA (The Modern Language Association) Style

Nabil, Nehal M., and Ahlam E. Yonis. "Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens." Alexandria Journal of Veterinary Sciences 62.2 (2019), 26-36. Print. doi:10.5455/ajvs.57274



APA (American Psychological Association) Style

Nabil, N. M. & Yonis, . A. E. (2019) Isolation of Salmonella Characterized By Biofilm Formation and Disinfectant Resistance From Broiler Chickens. Alexandria Journal of Veterinary Sciences, 62 (2), 26-36. doi:10.5455/ajvs.57274





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