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Original Research (Original Article) 


Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows

Hossain Ferdaus Mohd Altaf, Ara Anjuman, Rahman Md Mahfujur, Ilyas Nabila, Badruzzaman ATM, Zahran Eman, Hossain Md Mukter, Zinnah Mohammad Ali, Akhanda Mrs Rubaiat Nazneen, Islam Md Ashraful, Rahman Md Masudur1.

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  • Abstract
    Subclinical mastitis (SCM) is a major problem in the dairy industry creating a great loss both in quantity and quality of milk. The aim of this study to characterize bacteria, antibiogram profile, evaluation of immune cells and proinflammatory cytokines (IL-2, IL-6, TNF-α) associated with the inflammatory response in SCM. Out of total twenty six (26) milking cows, microbial and biochemical tests revealed that fifteen (15) cows (57.68%) having a total of 18 bacterial isolates of which the prevalence of coagulase negative Staphylococci spp., Staphylococcus aureus, Streptococci spp., E. coli, and Corynebacterium spp. were revealed as 38.89%, 16.67%, 22.22%, 16.67%, and 5.56%, respectively. On antibiogram profile, it was found effective against isolated organisms in ranking order are chlortetracycline, bacitracin, chloramphenicol, amoxicillin, nalidixic acid, penicillin, ampicillin, etc. although resistant isolates to penicillin, bacitracin, ampicillin, and chloramphenicol were detected. Blood and milk samples taken from those SCM+ cows further underwent for characterizing the pro-inflammatory cytokines IL-2, IL-6, and TNF-α, where both IL-2 and IL-6 revealed significant differences(P < 0.05); and then justified with the SCC, differential leucocytes differentiations. SCM+ milk and blood samples revealed a significantly higher (P < 0.05) level of total leukocytes and a marked neutrophilia prevailed in SCM+ cows than those of healthy ones. However, there were no significant differences found in aspect of macrophages, eosinophils, and lymphocytes population. Blood and milk albumen, but not globulin, showing significant differences (P < 0.05) in SCM cow blood and milk compared to control cows. Therefore, our findings suggest that microbes related with SCM cases results in increase pattern of neutrophilic inflammation and subsequent induction of pro-inflammatory cytokines.

    Key words: subclinical mastitis, crossbred cows, bacterial isolates, antibiogram profile, pro-inflammatory cytokines,


     
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    Pubmed Style

    Altaf HFM, Anjuman A, Mahfujur RM, Nabila I, ATM B, Eman Z, Mukter HM, Ali ZM, Nazneen AMR, Ashraful IM, Masudur RM. Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. AJVS. 2019; 62(2): 1-10. doi:10.5455/ajvs.58885


    Web Style

    Altaf HFM, Anjuman A, Mahfujur RM, Nabila I, ATM B, Eman Z, Mukter HM, Ali ZM, Nazneen AMR, Ashraful IM, Masudur RM. Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. http://www.alexjvs.com/?mno=58885 [Access: February 08, 2020]. doi:10.5455/ajvs.58885


    AMA (American Medical Association) Style

    Altaf HFM, Anjuman A, Mahfujur RM, Nabila I, ATM B, Eman Z, Mukter HM, Ali ZM, Nazneen AMR, Ashraful IM, Masudur RM. Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. AJVS. 2019; 62(2): 1-10. doi:10.5455/ajvs.58885



    Vancouver/ICMJE Style

    Altaf HFM, Anjuman A, Mahfujur RM, Nabila I, ATM B, Eman Z, Mukter HM, Ali ZM, Nazneen AMR, Ashraful IM, Masudur RM. Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. AJVS. (2019), [cited February 08, 2020]; 62(2): 1-10. doi:10.5455/ajvs.58885



    Harvard Style

    Altaf, H. F. M., Anjuman, . A., Mahfujur, . R. M., Nabila, . I., ATM, . B., Eman, . Z., Mukter, . H. M., Ali, . Z. M., Nazneen, . A. M. R., Ashraful, . I. M. & Masudur, . R. M. (2019) Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. AJVS, 62 (2), 1-10. doi:10.5455/ajvs.58885



    Turabian Style

    Altaf, Hossain Ferdaus Mohd, Ara Anjuman, Rahman Md Mahfujur, Ilyas Nabila, Badruzzaman ATM, Zahran Eman, Hossain Md Mukter, Zinnah Mohammad Ali, Akhanda Mrs Rubaiat Nazneen, Islam Md Ashraful, and Rahman Md Masudur. 2019. Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. Alexandria Journal of Veterinary Sciences, 62 (2), 1-10. doi:10.5455/ajvs.58885



    Chicago Style

    Altaf, Hossain Ferdaus Mohd, Ara Anjuman, Rahman Md Mahfujur, Ilyas Nabila, Badruzzaman ATM, Zahran Eman, Hossain Md Mukter, Zinnah Mohammad Ali, Akhanda Mrs Rubaiat Nazneen, Islam Md Ashraful, and Rahman Md Masudur. "Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows." Alexandria Journal of Veterinary Sciences 62 (2019), 1-10. doi:10.5455/ajvs.58885



    MLA (The Modern Language Association) Style

    Altaf, Hossain Ferdaus Mohd, Ara Anjuman, Rahman Md Mahfujur, Ilyas Nabila, Badruzzaman ATM, Zahran Eman, Hossain Md Mukter, Zinnah Mohammad Ali, Akhanda Mrs Rubaiat Nazneen, Islam Md Ashraful, and Rahman Md Masudur. "Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows." Alexandria Journal of Veterinary Sciences 62.2 (2019), 1-10. Print. doi:10.5455/ajvs.58885



    APA (American Psychological Association) Style

    Altaf, H. F. M., Anjuman, . A., Mahfujur, . R. M., Nabila, . I., ATM, . B., Eman, . Z., Mukter, . H. M., Ali, . Z. M., Nazneen, . A. M. R., Ashraful, . I. M. & Masudur, . R. M. (2019) Characterization of Bacterial Isolates, Antibiogram Profile and Pro-Inflammatory Cytokines in Subclinical Mastitis in Cross-Bred Dairy Cows. Alexandria Journal of Veterinary Sciences, 62 (2), 1-10. doi:10.5455/ajvs.58885





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