E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City

Heba S. Amin, Helmy A. Torky, Sawsan Kh. Ebied.

Abstract
The present study was conducted for molecular characterization of Salmonella Serovars that isolated from 200 samples including 100 food samples of animal origin (chicken meat, Chicken liver, Minced meat and meat products) purchased from retail stores , street vendors and supermarkets, 50 hand swab samples from handlers in retail stores or markets and 50 human diarrhaeic stool samples were collected from several hospital located in Alexandria province, Egypt. Samples were examined bacteriologically according to ISO 6579:2002 Al: 2007. Then confirmed by biochemical tests, serotyping, and molecular characterization.
The overall proportion of Salmonella positive was 4% (8/200) represented as 3%, 2%, and 2% from food samples, hand swab and diarrhoeic stool samples, respectively. Salmonella isolates were serologically typed as S. Enteritidis, S. Kentucky, and S. Tennesee. Also, Polymerase chain reaction (PCR) was carried out to detected invA (universal gene) and stn (Enterotoxin genes) for the Salmonella serovars , all Salmonella isolates were positive for (invA), while 87.5% were positive for (stn). Antibiogram profile for all Sallmonella isolates were carried out showing presence of multidrug resistance , all of the 8 Salmonella isolates tested (100%) were resistant to gentamicin, amikacin, cefuroxime and cefuroxime axetil.Also both S. Kentucky and S. Enteritidis (87.5%) isolated from food and human sample were rsistant to ampicillin, titarcyclin and trimethoprim/sulfamethoxazole. Two Salmonella isolates(S. Kentucky and S. Enteritidis) were isolated from human samples (25%) were resistant to ciprofloxacin and moxifloxacin.Only S. Kentucky (12.5%) isolated from human samples were resistant to cefotaxime ,cefodoxime, and intermediate to tigecyclin .

Key words: . Enteritidis, S. Kentucky, S. Tennesee , conventional PCR ,inva gene, stn gene, Antibiogram, Multidrug resistant.


 
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How to Cite this Article
Pubmed Style

Amin HS, Torky HA, Ebied SK. A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. AJVS. 2021; 71(2): 19-28. doi:10.5455/ajvs.125368


Web Style

Amin HS, Torky HA, Ebied SK. A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. http://www.alexjvs.com/?mno=125368 [Access: December 25, 2021]. doi:10.5455/ajvs.125368


AMA (American Medical Association) Style

Amin HS, Torky HA, Ebied SK. A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. AJVS. 2021; 71(2): 19-28. doi:10.5455/ajvs.125368



Vancouver/ICMJE Style

Amin HS, Torky HA, Ebied SK. A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. AJVS. (2021), [cited December 25, 2021]; 71(2): 19-28. doi:10.5455/ajvs.125368



Harvard Style

Amin, H. S., Torky, . H. A. & Ebied, . S. K. (2021) A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. AJVS, 71 (2), 19-28. doi:10.5455/ajvs.125368



Turabian Style

Amin, Heba S., Helmy A. Torky, and Sawsan Kh. Ebied. 2021. A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. Alexandria Journal of Veterinary Sciences, 71 (2), 19-28. doi:10.5455/ajvs.125368



Chicago Style

Amin, Heba S., Helmy A. Torky, and Sawsan Kh. Ebied. "A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City." Alexandria Journal of Veterinary Sciences 71 (2021), 19-28. doi:10.5455/ajvs.125368



MLA (The Modern Language Association) Style

Amin, Heba S., Helmy A. Torky, and Sawsan Kh. Ebied. "A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City." Alexandria Journal of Veterinary Sciences 71.2 (2021), 19-28. Print. doi:10.5455/ajvs.125368



APA (American Psychological Association) Style

Amin, H. S., Torky, . H. A. & Ebied, . S. K. (2021) A Contribution to Characterization of Salmonella Serovars Recovered from Retail Food and Human Being in Alexandria City. Alexandria Journal of Veterinary Sciences, 71 (2), 19-28. doi:10.5455/ajvs.125368





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