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Original Article
Online Published: 15 Apr 2015
 


Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein.


Abstract
Rabbit viral haemorrhagic disease (RVHD) is a highly contagious, peracute and acute viral disease of both wild and domestic rabbits caused by rabbit haemorrhagic disease virus (RHDV). RHDV is considered as a haemagglutinating virus; however, there is a growing evidence for presence of non haemagglutinating RHDV strains. In this study, two strains of RHDV have been characterized. The two strains were selected from ten different infected rabbit flocks. One of them had haemagglutination (HA) activity and the other one lacked HA activity. HA test was carried out on both isolates at 4°C using human red blood cells (RBC) type ‘O’. RT-PCR was performed on the liver samples of the ten infected rabbit flocks using specific primers targeting VP60 gene. The obtained partial VP60 gene sequences of the two selected strains were aligned with other haemagglutinating and non haemagglutinating strains of RHDV in the world, including ‘‘RHDVa’’ strains as France 99-05, France-Reu-00, Germany-Triptis and China TP. Phylogenetic analysis showed that the two strains were genetically different. Haemagglutinating strain was similar to Rainham strain which is low haemagglutinating virus while non haemagglutinating strain was similar to whn-1 strain which is non haeagglutinating virus. The study confirmed presence of non haemagglutinating RHDV in rabbits reared in Egypt and suggested that VP60 might be related to HA activity.

Key words: Rabbit Hemorrhagic Disease Virus, haemagglutinating, Non-haemagglutinating, VP60 gene, Sequence analysis


 
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Pubmed Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. AJVS. 2015; 45(1): 1-5. doi:10.5455/ajvs.177331

Web Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. https://www.alexjvs.com/?mno=177331 [Access: December 01, 2024]. doi:10.5455/ajvs.177331

AMA (American Medical Association) Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. AJVS. 2015; 45(1): 1-5. doi:10.5455/ajvs.177331


Vancouver/ICMJE Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. AJVS. (2015), [cited December 01, 2024]; 45(1): 1-5. doi:10.5455/ajvs.177331


Harvard Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein (2015) Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. AJVS, 45 (1), 1-5. doi:10.5455/ajvs.177331


Turabian Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. 2015. Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. Alexandria Journal of Veterinary Sciences, 45 (1), 1-5. doi:10.5455/ajvs.177331


Chicago Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. "Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt." Alexandria Journal of Veterinary Sciences 45 (2015), 1-5. doi:10.5455/ajvs.177331


MLA (The Modern Language Association) Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein. "Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt." Alexandria Journal of Veterinary Sciences 45.1 (2015), 1-5. Print. doi:10.5455/ajvs.177331


APA (American Psychological Association) Style

Abdel Hameed I. Bazid, Mohammed M. AboElkhair, Alaa G. Abdel-Razik, Rania Salim, Hesham A. Sultan, Hussein A. Hussein (2015) Molecular Characterization of a Haemagglutinating and Non Haemagglutinating Rabbit Haemorrhagic Disease Viruses From Egypt. Alexandria Journal of Veterinary Sciences, 45 (1), 1-5. doi:10.5455/ajvs.177331




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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