E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article
Online Published: 04 Aug 2015
 


Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat

Helmy A Torky, Hanaa A Ahmed, Emad S Omran.


Abstract
In this study, duplex real-time PCR approach with melting curve analysis is presented for the detection of Listeria monocytogenes, which are important food-borne bacterial pathogens usually present in imported frozen meat. Melting curve analysis using a SYBR Green I real-time PCR approach showed characteristic Tm values demonstrating the specific and efficient amplification of the two pathogens;73.2°C and 81.54°C for L. monocytogenes and Salmonella spp., respectively, the duplex, real-time PCR was equally sensitive to uniplex real-time PCR, using same amounts of purified DNA. When our established duplex real-time PCR assay was applied to artificially inoculated imported frozen meat the detection limit was 103 CFU/g for each of these pathogens without enrichment. The overall process included the culture enrichment based on the ISO standard, consisting of 24 h incubation in appropriate media (Half Fraser Broth for Listeria and buffered peptone water (BPW) for Salmonella), followed by a single DNA extraction of mixed enrichment aliquot. The results from this study showed that the developed duplex real-time PCR with melting curve analysis is promising as a rapid and cost-effective test method for improving food safety.

Key words: Salmonella, Listeria monocytogenes, Multiplex, Real-time PCR


 
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How to Cite this Article
Pubmed Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. 2015; 46(1): 100-109. doi:10.5455/ajvs.185240

Web Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. https://www.alexjvs.com/?mno=185240 [Access: May 04, 2025]. doi:10.5455/ajvs.185240

AMA (American Medical Association) Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. 2015; 46(1): 100-109. doi:10.5455/ajvs.185240


Vancouver/ICMJE Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. (2015), [cited May 04, 2025]; 46(1): 100-109. doi:10.5455/ajvs.185240


Harvard Style

Torky, H. A., Ahmed, . H. A. & Omran, . E. S. (2015) Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS, 46 (1), 100-109. doi:10.5455/ajvs.185240


Turabian Style

Torky, Helmy A, Hanaa A Ahmed, and Emad S Omran. 2015. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. Alexandria Journal of Veterinary Sciences, 46 (1), 100-109. doi:10.5455/ajvs.185240


Chicago Style

Torky, Helmy A, Hanaa A Ahmed, and Emad S Omran. "Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat." Alexandria Journal of Veterinary Sciences 46 (2015), 100-109. doi:10.5455/ajvs.185240


MLA (The Modern Language Association) Style

Torky, Helmy A, Hanaa A Ahmed, and Emad S Omran. "Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat." Alexandria Journal of Veterinary Sciences 46.1 (2015), 100-109. Print. doi:10.5455/ajvs.185240


APA (American Psychological Association) Style

Torky, H. A., Ahmed, . H. A. & Omran, . E. S. (2015) Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. Alexandria Journal of Veterinary Sciences, 46 (1), 100-109. doi:10.5455/ajvs.185240




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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