E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article
Online Published: 04 Apr 2024
 


Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens

Mary M. Hana, Eman E. Abdeen.


Abstract
Pseudomonas aeruginosa (P. aeruginosa) is considered an opportunistic pathogen that causes high morbidity and mortality and is responsible for a high proportion of hospital infections pathogen that can cause several disease syndromes in the community and hospitals. Recently, P. aeruginosa infections having antibiotic resistance have been increasing and serious problems are being experienced in treatment. This study aimed to determine the prevalence, antibiogram pattern, and molecular identification of oprL virulence gene of P. aeruginosa recovered from human pus. One hundred samples from human pus were collected for bacteriological identification before being subjected to molecular identification. The results revealed that 13/100 (13%) were positive for P. aeruginosa on Citramide ager. All isolates showed high sensitivity to Imipenem, Apramycin, Amikacin, Norfloxacin, Sulphamethoxazol, with 100%, 92.31%, 84.61%, 9.23%, and 61.53% respectively. For the molecular characterization of P. aeruginosa (5 isolates) for detection of oprL virulence gene. The results indicated that oprL genes were the detected genes in all tested samples with (100%).In conclusion; the obtained results provide important data about the importance of the oprL virulence gene and pathogenicity of P. aeruginosa as well as the widespread of MDR strains that complicated the treatment options in human community

Key words: P. aeruginosa, antibiogram, Virulence, human, infection


 
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How to Cite this Article
Pubmed Style

Hana MM, Abdeen EE. Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. AJVS. 2024; 81(0): 19-26. doi:10.5455/ajvs.193630


Web Style

Hana MM, Abdeen EE. Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. https://www.alexjvs.com/?mno=193630 [Access: September 13, 2024]. doi:10.5455/ajvs.193630


AMA (American Medical Association) Style

Hana MM, Abdeen EE. Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. AJVS. 2024; 81(0): 19-26. doi:10.5455/ajvs.193630



Vancouver/ICMJE Style

Hana MM, Abdeen EE. Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. AJVS. (2024), [cited September 13, 2024]; 81(0): 19-26. doi:10.5455/ajvs.193630



Harvard Style

Hana, M. M. & Abdeen, . E. E. (2024) Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. AJVS, 81 (0), 19-26. doi:10.5455/ajvs.193630



Turabian Style

Hana, Mary M., and Eman E. Abdeen. 2024. Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. Alexandria Journal of Veterinary Sciences, 81 (0), 19-26. doi:10.5455/ajvs.193630



Chicago Style

Hana, Mary M., and Eman E. Abdeen. "Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens." Alexandria Journal of Veterinary Sciences 81 (2024), 19-26. doi:10.5455/ajvs.193630



MLA (The Modern Language Association) Style

Hana, Mary M., and Eman E. Abdeen. "Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens." Alexandria Journal of Veterinary Sciences 81.0 (2024), 19-26. Print. doi:10.5455/ajvs.193630



APA (American Psychological Association) Style

Hana, M. M. & Abdeen, . E. E. (2024) Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens. Alexandria Journal of Veterinary Sciences, 81 (0), 19-26. doi:10.5455/ajvs.193630





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