E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
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Original Article
Online Published: 18 Apr 2016
 


Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil.


Abstract
To evaluate the current situation of infection with EDS-76virus in Egypt, a total of 35 field samples (15 kidneys, 16 oviduct and 4 oviduct swabs) were collected from 10 layer flocks raised in Menoufia, El-Behera and Gharbia governorates during the period 2014-2015. The flocks showed a decreased laying performance associated with the production of soft-shelled eggs. Virus isolation was attempted in 10-day-old embryonated duck eggs via allantoic sac inoculation.Five days after inoculation, the allantoic fluids were tested for the presence of hemagglutinating (HA) activity using chicken erythrocytes. Seven samples were tested positive for HA activity throughout three consecutive passages.The presence of EDS -76 virus was assessed in all HA-positive allantoic fluids by hexon gene-based conventional and real-time PCR assays. In 6 out of 7 samples, PCR was successfully applied to detect the virus DNA, meanwhile all the employed samples were tested positive for the presence of EDS-76 virus by real-time PCR assay. The SYBR green-based real-time PCR assay described in the present study was found to be more sensitive and less time consuming than conventional PCR assay for detection of EDS-76 virus genome.

Key words: Egg drop syndrome 76 virus, real-time PCR assay, laying hens, Egypt.


 
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Pubmed Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. AJVS. 2016; 49(1): 55-59. doi:10.5455/ajvs.211755

Web Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. https://www.alexjvs.com/?mno=211755 [Access: December 04, 2024]. doi:10.5455/ajvs.211755

AMA (American Medical Association) Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. AJVS. 2016; 49(1): 55-59. doi:10.5455/ajvs.211755


Vancouver/ICMJE Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. AJVS. (2016), [cited December 04, 2024]; 49(1): 55-59. doi:10.5455/ajvs.211755


Harvard Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil (2016) Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. AJVS, 49 (1), 55-59. doi:10.5455/ajvs.211755


Turabian Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. 2016. Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. Alexandria Journal of Veterinary Sciences, 49 (1), 55-59. doi:10.5455/ajvs.211755


Chicago Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. "Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt." Alexandria Journal of Veterinary Sciences 49 (2016), 55-59. doi:10.5455/ajvs.211755


MLA (The Modern Language Association) Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil. "Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt." Alexandria Journal of Veterinary Sciences 49.1 (2016), 55-59. Print. doi:10.5455/ajvs.211755


APA (American Psychological Association) Style

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil (2016) Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt. Alexandria Journal of Veterinary Sciences, 49 (1), 55-59. doi:10.5455/ajvs.211755




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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