E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
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Original Article
Online Published: 03 Dec 2024
 


An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals

Asmaa Elsayed Gamal Eldeen, rania Saleh Mahmoud, abdelhalim Abdelaty Elshawadfy, attia Abd Allah Elgedawy.


Abstract
Mycobacterium avium subspecies paratuberculosis(MAP) is one of the most infectious bacteria affecting small and large ruminants causing Johne's disease which is characterized by chronic diarrhea , severe emaciation and decreasing of the milk production leading to highly effective economic losses. Also, MAP causes Johne's disease in human. In this study, blood without anticoagulant (n=200) and faecal (n=200) samples were collected from sheep, goats, cattle and buffaloes. By using of ZN stain, smears from decontaminated faecal samples revealed presence of acid fast bacilli (68%). Serum samples separated from blood tested by ELISA showed the presence of antibodies against MAP in sheep (20%), goats (17.1%), cattle (22.5%), and buffaloes (16%). Faecal samples were tested by PCR for detection of IS900 specific gene for confirmation of MAP strains and it was detected in (26.7%) sheep, (25.7%) goats, (28.8%) cattle, and (20%) buffaloes. Also, the conventional PCR was used to differentiate between 2 types of MAP where S- type was detected in (9)sheep, (5) goats, (12) cattle, and (3) buffaloes and C- type was detected in (7) sheep, (4) goats, (11) cattle, and (2) buffaloes. This study proved that MAP is a widespread bacterium and shows the highly spreading of its strains in ruminants.

Key words: : MAP, Johne's disease, Ruminants, ELISA, PCR


 
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How to Cite this Article
Pubmed Style

Eldeen AEG, Mahmoud rS, Elshawadfy aA, Elgedawy aAA. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS. Online First: 03 Dec, 2024.

Web Style

Eldeen AEG, Mahmoud rS, Elshawadfy aA, Elgedawy aAA. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. https://www.alexjvs.com/?mno=226337 [Access: December 06, 2024].

AMA (American Medical Association) Style

Eldeen AEG, Mahmoud rS, Elshawadfy aA, Elgedawy aAA. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS. Online First: 03 Dec, 2024.


Vancouver/ICMJE Style

Eldeen AEG, Mahmoud rS, Elshawadfy aA, Elgedawy aAA. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS, [cited December 06, 2024]; Online First: 03 Dec, 2024.


Harvard Style

Eldeen, A. E. G., Mahmoud, . r. S., Elshawadfy, . a. A. & Elgedawy, . a. A. A. (0) An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS, Online First: 03 Dec, 2024.


Turabian Style

Eldeen, Asmaa Elsayed Gamal, rania Saleh Mahmoud, abdelhalim Abdelaty Elshawadfy, and attia Abd Allah Elgedawy. 0. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. Alexandria Journal of Veterinary Sciences, Online First: 03 Dec, 2024.


Chicago Style

Eldeen, Asmaa Elsayed Gamal, rania Saleh Mahmoud, abdelhalim Abdelaty Elshawadfy, and attia Abd Allah Elgedawy. "An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals." Alexandria Journal of Veterinary Sciences Online First: 03 Dec, 2024.


MLA (The Modern Language Association) Style

Eldeen, Asmaa Elsayed Gamal, rania Saleh Mahmoud, abdelhalim Abdelaty Elshawadfy, and attia Abd Allah Elgedawy. "An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals." Alexandria Journal of Veterinary Sciences Online First: 03 Dec, 2024. Web. 06 Dec 2024


APA (American Psychological Association) Style

Eldeen, A. E. G., Mahmoud, . r. S., Elshawadfy, . a. A. & Elgedawy, . a. A. A. (0) An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. Alexandria Journal of Veterinary Sciences, Online First: 03 Dec, 2024.




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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