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Original Article
Online Published: 11 Aug 2016
 


Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef.


Abstract
A pool of brain sample was collected from three pigeons showing torticollis and whitish green diarrhea in Desouk, Kafr-elshiekh Province, Egypt,during January, 2014. Virus Isolation has been pursued by inoculation of brain homogenate into the allantoic sac of 9 day old specific pathogen free (SPF) embryonated chicken eggs and incubated at 37oC for 4 days. Identification of the isolate was carried out by haemagglutination inhibition test using specific polyclonal antisera against NDV and AIV ( subtype H5 and H9), RT-PCR and confirmed by sequencing. Partial deduced amino acid sequence of the fusion protein at the cleavage site revealed that the isolate possessed the motif 112 K-R-Q-K-R-F117 indicating velogenic entity and phylogenetic analysis showed clustering of the study sequence with other genotype IV F gene sequences available in GenBank.The study indicated that pigeons may play epidemiological role in the introduction of a new NDV genotype VI to commercials chicken.

Key words: Newcastle disease virus; Velogenic; pigeon; Genotype IV; Egypt


 
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How to Cite this Article
Pubmed Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. 2016; 50(1): 145-149. doi:10.5455/ajvs.230284

Web Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. https://www.alexjvs.com/?mno=230284 [Access: December 01, 2024]. doi:10.5455/ajvs.230284

AMA (American Medical Association) Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. 2016; 50(1): 145-149. doi:10.5455/ajvs.230284


Vancouver/ICMJE Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. (2016), [cited December 01, 2024]; 50(1): 145-149. doi:10.5455/ajvs.230284


Harvard Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef (2016) Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS, 50 (1), 145-149. doi:10.5455/ajvs.230284


Turabian Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. 2016. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. Alexandria Journal of Veterinary Sciences, 50 (1), 145-149. doi:10.5455/ajvs.230284


Chicago Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. "Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt." Alexandria Journal of Veterinary Sciences 50 (2016), 145-149. doi:10.5455/ajvs.230284


MLA (The Modern Language Association) Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef. "Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt." Alexandria Journal of Veterinary Sciences 50.1 (2016), 145-149. Print. doi:10.5455/ajvs.230284


APA (American Psychological Association) Style

Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef (2016) Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. Alexandria Journal of Veterinary Sciences, 50 (1), 145-149. doi:10.5455/ajvs.230284




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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