Abstract
Sarcocystis causes Poor feed efficiency in intermediate hosts, leading to emaciation, fever, and an increase in the pH of the meat, all of which contribute to low-quality meat and Insufficient marketability. Some species of Sarcocystis are Zoonotic to human As S.hominis. This study included 120 randomly selected samples from the oesophagus and various muscles, including the diaphragm, masseter, tongue, and heart of cows and buffalos (50 randomly selected samples from cattle and 70 randomly selected samples from buffalo). The samples were gathered from various locations around Egypt, including the abattoirs in East and West Alexandria, Al-Boheira, Cairo, and Giza. Macroscopic parasitological examination was used to identify the macroscopic form of Sarcocystis spp. in the carcasses of cattle and buffaloes. This was followed by microscopic examination of Sarcocystis spp. using a trichinoscope, which was the most rapid and easiest method, peptic digestion for the most accurate method of detecting bradyzoites, and histopathological technique for identifying cysts and eosinophils linked to Bovine Eosinophilic Myositis (BEM). It was found that the prevalence of macroscopic sarcocystis in buffaloes was 48.57% (S. fusiformis) While in cattle was zero. Oesophagus is the most infected organ in carcass of buffalo with S.fusiformis 35.71% Followed by masseter muscle 7.14%. While microscopic sarcocystis in buffaloes 88.57% and in cattle 60% Oesophagus and masseter muscle are the most infected organs with microscopic form of sarcocystis in both cattle and buffaloes. The spring has the highest prevalence with a macroscopic sarcocystis (56%) in buffaloes. Macroscopic sarcocystis in buffaloes is more common in females than in males and in old than young. pH values were determined using meat samples in order to investigate the impact of sarcocystis on meat quality.

Key words: Sarcocystis, Trichinoscope, Egypt, Myositis, pH