The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis

Doaa Kirat

doi:10.5455/ajvs.242177

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Original Article

AJVS. 2016; 51(1): 61-67

doi: 10.5455/ajvs.242177

The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis

Doaa Kirat.

Abstract
The present work was designed to study the effect of metabolic acidosis induced by ammonium chloride on the expression of sodium-bicarbonate cotransporter 1 (NBC1) and Monocarboxylate transporter 1 (MCT1) in rat kidney. Following the treatment of NH4Cl, blood pH and bicarbonate concentration were significantly decreased, while significant increase in chloride concentration was developed. Moreover, urine analysis revealed decrease in urine pH and significant increase in ammonia in acidotic rats. The protein expression of NBC1 and MCT1 was determined in the rat kidney by immunofluorescence confocal laser-scanning microscopy and Western blotting analyses. In the experimental group, the expression level of NBC1 was significantly increased in the renal proximal tubules, whereas, the expression of MCT1 was significantly decreased. Western blotting data confirmed the immunofluorescence confocal laser-scanning microscopy. In conclusion, metabolic acidosis causes adaptive increases in the basolateral NBC1 in the rat renal proximal tubule that are likely responsible for the increased ability of the proximal tubule to reabsorb filtered bicarbonates. Additionally, acidosis leads to decrease the MCT1 protein expression level in renal proximal tubule that may explain the increase in lactate excretion in the urine of acidotic rats due to the reduction in lactate co-transport.

Key words: Monocarboxylate transporter 1, Sodium- bicarbonate cotransporter 1, Ammonium Chloride, Kidney, Rats.

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Pubmed Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. 2016; 51(1): 61-67. doi:10.5455/ajvs.242177

Web Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. https://www.alexjvs.com/?mno=242177 [Access: May 04, 2025]. doi:10.5455/ajvs.242177

AMA (American Medical Association) Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. 2016; 51(1): 61-67. doi:10.5455/ajvs.242177

Vancouver/ICMJE Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. (2016), [cited May 04, 2025]; 51(1): 61-67. doi:10.5455/ajvs.242177

Harvard Style

Doaa Kirat (2016) The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS, 51 (1), 61-67. doi:10.5455/ajvs.242177

Turabian Style

Doaa Kirat. 2016. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. Alexandria Journal of Veterinary Sciences, 51 (1), 61-67. doi:10.5455/ajvs.242177

Chicago Style

Doaa Kirat. "The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis." Alexandria Journal of Veterinary Sciences 51 (2016), 61-67. doi:10.5455/ajvs.242177

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APA (American Psychological Association) Style

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