E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article
Online Published: 07 Jan 2017
 


Molecular Characterization of Vibrio Harveyi in Diseased Shrimp

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem.


Abstract
The objective of this study was to characterize Vibrio harveyi phenotypically and by molecular methods. A total number of 420 shrimp post larvae samples were collected, 280 samples of diseased cultured shrimp and 140 samples of apparently healthy marine shrimp. The samples were subjected to bacteriological examination. Thirty three (11.7%) of cultured shrimp samples and 4 (2.8%) of marine shrimp samples were phenotypically positive for V. harveyi. Species - specific gene (toxRgene) and two virulence associated genes (vhh -Vibrio harveyi haemolysin, and partial hly- haemolysin gene) were investigated using conventional PCR.
This study proved that using of partial haemolysin gene in molecular characterization of V. harveyi is the fastest and most accurate method to identify all isolates of V. harveyi (highly pathogenic, moderately pathogenic and non-pathogenic strains) due to the presence of a single copy of haemolysin gene encoded in all isolates, although it is not in the same locus in the genome.

Key words: Molecular Characterization, Vibrio harveyi, Diseased Shrimp.


 
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How to Cite this Article
Pubmed Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029

Web Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. https://www.alexjvs.com/?mno=255141 [Access: March 25, 2025]. doi:10.5455/ajvs.206029

AMA (American Medical Association) Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029


Vancouver/ICMJE Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. (2016), [cited March 25, 2025]; 51(2): 358-366. doi:10.5455/ajvs.206029


Harvard Style

Torky, H. A., Abdellrazeq, . G. S., Hussein, . M. M. & Ghanem, . N. H. (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS, 51 (2), 358-366. doi:10.5455/ajvs.206029


Turabian Style

Torky, Helmy A., Gaber S. Abdellrazeq, Mona M. Hussein, and Nourhan H. Ghanem. 2016. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029


Chicago Style

Torky, Helmy A., Gaber S. Abdellrazeq, Mona M. Hussein, and Nourhan H. Ghanem. "Molecular Characterization of Vibrio Harveyi in Diseased Shrimp." Alexandria Journal of Veterinary Sciences 51 (2016), 358-366. doi:10.5455/ajvs.206029


MLA (The Modern Language Association) Style

Torky, Helmy A., Gaber S. Abdellrazeq, Mona M. Hussein, and Nourhan H. Ghanem. "Molecular Characterization of Vibrio Harveyi in Diseased Shrimp." Alexandria Journal of Veterinary Sciences 51.2 (2016), 358-366. Print. doi:10.5455/ajvs.206029


APA (American Psychological Association) Style

Torky, H. A., Abdellrazeq, . G. S., Hussein, . M. M. & Ghanem, . N. H. (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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