Abstract
Rumenal fistula is very essential in nutrition and physiology experimental research studies in sheep. Permanent placement of the cannula without complication is a challenge. This experimental study aimed to provide a detailed description of modified one-stage technique for rumenal fistula in sheep to simplify the surgery, decrease the post-operative complications, and lengthen the functional life of the cannula. As well as reporting the postoperative complications related to the surgery as well as short and long term monitoring. 10 healthy rams were surgically prepared for left side laparotomy in lateral recumbent position. The rumen was grasped, anchored to the subcutaneous tissue, and incised to fit the custom-made cannula. The rumenal wall was sutured directly to the skin after insertion of the cannula, and a metal retainer flange was pushed to secure the cannula in place. No early post-surgical complications. None fistula got a bigger size, and none cannula was rejected, or had to be repositioned. The one-stage technique used in this study to fix a custom-made cannula in its position recommended to experimental research on sheep nutrition and physiology.

Key words: Sheep, rumenal fistula, one-stage technique.