E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 13 Feb 2017
 


Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine

Ahmed Samy1, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar.


Abstract
Clostridium Perfringens represent one of the natural inhabitant bacteria in the gastrointestinal tract of chickens. However, predisposing factors, mainly co-infection with Coccidia, represent a favorable condition for over growth of C. perfringens that induce necrotic enteritis (NE). The prevalence of C. perfringens in commercial broilers with enteric disease in Egypt was investigated via bacteriological isolation and identification and then confirmed by toxin typing by PCR. Furthermore, culture-independent quantitative real time PCR targeting alpha toxin gene was improved via reducing the effect of PCR inhibitors in the intestinal content (IC). Out of 61 broiler flocks, 40 were positive for C. perfringens with a higher incidence in winter (81.25%) and in 20-30 days old broilers (66.67%). All isolates belonged to C. perfringens type A with the absence of enterotoxin or Beta2 toxin genes. Pretreatment of the intestinal content with acetone improve the detection limit of quantitative real time PCR assay to 102 cells/gm which was highly correlated to the culture-based counting method using cooked meet media. C. perfringes type A widespread in broilers in Egypt and the extraction method used herein could be used for direct quantification of C. perfringens in the intestinal content of broiler chickens without prior enrichment.

Key words: Clostridium perfringens, Broilers, intestinal inhibitors, Necrotic Enteritis, Quantitative Real-Time PCR.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ahmed Samy1
Articles by Soad A.Nasef
Articles by May F. Abdelaty
Articles by Ashraf M. Abdelwahab
Articles by Gamal A Amer and Ahmed M. Ammar
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . AJVS. 2017; 52(1): 160-165. doi:10.5455/ajvs.250693

Web Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . https://www.alexjvs.com/?mno=258476 [Access: September 12, 2024]. doi:10.5455/ajvs.250693

AMA (American Medical Association) Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . AJVS. 2017; 52(1): 160-165. doi:10.5455/ajvs.250693


Vancouver/ICMJE Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . AJVS. (2017), [cited September 12, 2024]; 52(1): 160-165. doi:10.5455/ajvs.250693


Harvard Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar (2017) Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . AJVS, 52 (1), 160-165. doi:10.5455/ajvs.250693


Turabian Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. 2017. Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . Alexandria Journal of Veterinary Sciences, 52 (1), 160-165. doi:10.5455/ajvs.250693


Chicago Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. "Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine ." Alexandria Journal of Veterinary Sciences 52 (2017), 160-165. doi:10.5455/ajvs.250693


MLA (The Modern Language Association) Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar. "Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine ." Alexandria Journal of Veterinary Sciences 52.1 (2017), 160-165. Print. doi:10.5455/ajvs.250693


APA (American Psychological Association) Style

Ahmed Samy, Soad A.Nasef, May F. Abdelaty, Ashraf M. Abdelwahab, Gamal A Amer and Ahmed M. Ammar (2017) Prevalence and Improvement of Culture Independent Quantification of Clostridium Perfringens in Broiler’s Intestine . Alexandria Journal of Veterinary Sciences, 52 (1), 160-165. doi:10.5455/ajvs.250693




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine, Alexandria University
    Edfina, Behaira, Egypt.
    PO.Box: 22758
    Telefax: +20 45 2963526
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use