E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
84Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah.


Abstract
In this study, a total number of animal samples (583) were collected from cattle (170) , sheep (221) and goats (192) suffering from respiratory manifestations (freshly dead and slaughtered). Nasal swabs (169) were collected from cattle (43), sheep (67) and goats (59) . Blood samples (312) were collected from cattle (95) , sheep (112) and goats (105) and lung and spleen tissues (102) were collected from cattle (32) ,sheep (42) and goats (28) . Samples collected for bacteriological isolation and identification of P. multocida. After that confirmation of identification by using PM-PCR, and multiplex-PCR for detection of the virulence factors genes, DNA nucleotides sequencing of these genes were done for detection of the genetic diversity.
The prevalence of P.multocida isolated from all samples from Cattle, Sheep and Goats is 1.76%, 1.8%, 1.56% respectively. While, P. multocida not found in all blood samples collected from cattle, sheep and goat.
PM-PCR and multiplex-PCR confirmed presence of genes under examination.
DNA nucleotide sequencing and analysis of sequences obtained for the 5 genes under investigation Concluded that: P. multocida isolates had no genetic diversity of oma 87 gene, ptf A gene, phf A, tox A and nan B among cattle, sheep and goats.

Key words: Genetic diversity, Pasteurella multocida, virulence factors genes.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Samy A. Khalil
Articles by Ahmed A. Bkheet
Articles by Fayez A. Gad Allah
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961

Web Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. https://www.alexjvs.com/?mno=263961 [Access: December 02, 2024]. doi:10.5455/ajvs.263961

AMA (American Medical Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961


Vancouver/ICMJE Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. (2017), [cited December 02, 2024]; 54(1): 173-178. doi:10.5455/ajvs.263961


Harvard Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS, 54 (1), 173-178. doi:10.5455/ajvs.263961


Turabian Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. 2017. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961


Chicago Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. "Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species." Alexandria Journal of Veterinary Sciences 54 (2017), 173-178. doi:10.5455/ajvs.263961


MLA (The Modern Language Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. "Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species." Alexandria Journal of Veterinary Sciences 54.1 (2017), 173-178. Print. doi:10.5455/ajvs.263961


APA (American Psychological Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use