E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby.


Abstract
The aim of this study is to screen for genetic resistance to different doses of salmonella infection and its possible association with polymorphisms in exon 1 and 2 of TLR4 in Fayoumi breed and Hy-line strain of chickens. Two experiments was done, first one for measuring the variation of TLR4 -exon 1 and exon 2 of different genotypes of chicken under study and resistance to salmonella enteritidis. This study include 100 birds of each breeds, were infected orally with LD50 (108 cfu) salmonella enteritidis at age of two weeks and was divided into two groups (susceptible and resistance). TLR4 gene was genotyped with PCR-RFLP, The genotyping results on exon 1 (596 bp) by Taq1 restriction enzyme showed that the BB genotypes is more resistance to infection in the Fayoumi breed and also the AB genotypes is more resistance to infection in the Hy-line strain. Also The genotyping result on exon 2 (793 bp) by EcoR1 restriction enzyme showed only one genotype BB in Fayoumi breed and The frequency of AB genotypes is more resistant to infection than BB genotypes in Hy-line strain. The second experiment to evaluate the variation in immune response to the different genotypes of TLR4 -exon 1 and exon 2 this study include 100 birds of each breeds were infected with (5× 107 cfu) salmonella enteritidis and according to Elisa results the chickens devided into two groups (high and low immune response). The genotyping result on exon 1 (596 bp) by Taq1 restriction enzyme showed that the AB genotypes produce high immune response in both Fayoumi breed and Hy-line strain so can be used as a genetic marker for increase the immune response in chicks under study. Also The genotyping result on exon 2 (793 bp) by EcoR1 restriction enzyme showed only one genotype BB genotypes in Fayoumi breed and Hy-line strain.

Key words: Tol-like receptor, polymorphism, genetic resistance, salmonella enteritidis chicken


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Shymaa A. Khatab
Articles by Shabaan A. Hemeda
Articles by Abeer F. El-Nahas
Articles by Walaa S.H. Abd El Naby
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. AJVS. 2017; 55(2): 1-9. doi:10.5455/ajvs.273717

Web Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. https://www.alexjvs.com/?mno=273717 [Access: December 02, 2024]. doi:10.5455/ajvs.273717

AMA (American Medical Association) Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. AJVS. 2017; 55(2): 1-9. doi:10.5455/ajvs.273717


Vancouver/ICMJE Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. AJVS. (2017), [cited December 02, 2024]; 55(2): 1-9. doi:10.5455/ajvs.273717


Harvard Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby (2017) Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. AJVS, 55 (2), 1-9. doi:10.5455/ajvs.273717


Turabian Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. 2017. Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. Alexandria Journal of Veterinary Sciences, 55 (2), 1-9. doi:10.5455/ajvs.273717


Chicago Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. "Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt." Alexandria Journal of Veterinary Sciences 55 (2017), 1-9. doi:10.5455/ajvs.273717


MLA (The Modern Language Association) Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby. "Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt." Alexandria Journal of Veterinary Sciences 55.2 (2017), 1-9. Print. doi:10.5455/ajvs.273717


APA (American Psychological Association) Style

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby (2017) Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt. Alexandria Journal of Veterinary Sciences, 55 (2), 1-9. doi:10.5455/ajvs.273717




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use