E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Ayman S. Nofal, Ahlam A. El-leboudy, Hussein S. Abo El-makarem, Amir H.abd El-rahman.


Abstract
Brucellosis is a worldwide zoonotic disease that causes major public health hazard in Egypt. The present work was conducted to investigate the prevalence of bovine brucellosis on the basis of serological test RBPT and MRT in serum and milk samples of lactating dairy animals, also isolation of brucella organism from milk samples, PCR amplification was done for detection of Brucella abortus DNA using BCSP31 target gene. 219 serum samples from sheep and goat and 119 milk samples from cow, sheep, and goat were collected from a governmental and private farm. The prevalence of brucellosis by RBPT was 34.5 and 61.4% in serum samples of sheep and goat also, by MRT were 2.3, 13.8 and 10% in milk samples of cow, sheep, and goat, respectively. Milk samples were cultured on the Brucella agar medium and gave the same prevalence rate. A specific PCR assay targeting the bcsp-31 gene within the genome of Brucella species has been used and gave an amplicon of 223 bp in three strain of isolated brucella. It is concluded that RBPT and MRT used as screening test for detection prevalence of brucella species in serum and milk serum, PCR assay consider a useful tool for detection Brucella species in milk.

Key words: Brucella species, RBPT, MRT, bcsp-31 gene


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ayman S. Nofal
Articles by Ahlam A. El-Leboudy
Articles by Hussein S. Abo El-Makarem
Articles by Amir H.Abd El-Rahman
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Nofal AS, El-leboudy AA, El-makarem HSA, El-rahman AH. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. 2017; 55(2): 19-24. doi:10.5455/ajvs.278394

Web Style

Nofal AS, El-leboudy AA, El-makarem HSA, El-rahman AH. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. https://www.alexjvs.com/?mno=278394 [Access: March 26, 2025]. doi:10.5455/ajvs.278394

AMA (American Medical Association) Style

Nofal AS, El-leboudy AA, El-makarem HSA, El-rahman AH. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. 2017; 55(2): 19-24. doi:10.5455/ajvs.278394


Vancouver/ICMJE Style

Nofal AS, El-leboudy AA, El-makarem HSA, El-rahman AH. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. (2017), [cited March 26, 2025]; 55(2): 19-24. doi:10.5455/ajvs.278394


Harvard Style

Nofal, A. S., El-leboudy, . A. A., El-makarem, . H. S. A. & El-rahman, . A. H. (2017) Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS, 55 (2), 19-24. doi:10.5455/ajvs.278394


Turabian Style

Nofal, Ayman S., Ahlam A. El-leboudy, Hussein S. Abo El-makarem, and Amir H.abd El-rahman. 2017. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. Alexandria Journal of Veterinary Sciences, 55 (2), 19-24. doi:10.5455/ajvs.278394


Chicago Style

Nofal, Ayman S., Ahlam A. El-leboudy, Hussein S. Abo El-makarem, and Amir H.abd El-rahman. "Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals." Alexandria Journal of Veterinary Sciences 55 (2017), 19-24. doi:10.5455/ajvs.278394


MLA (The Modern Language Association) Style

Nofal, Ayman S., Ahlam A. El-leboudy, Hussein S. Abo El-makarem, and Amir H.abd El-rahman. "Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals." Alexandria Journal of Veterinary Sciences 55.2 (2017), 19-24. Print. doi:10.5455/ajvs.278394


APA (American Psychological Association) Style

Nofal, A. S., El-leboudy, . A. A., El-makarem, . H. S. A. & El-rahman, . A. H. (2017) Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. Alexandria Journal of Veterinary Sciences, 55 (2), 19-24. doi:10.5455/ajvs.278394




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use