E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
84Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk

El Sayed F.H. Ibrahim, Ashraf M. Abd El wahab, Samy A. Khalil, Helmy A. Torky.


Abstract
Bacterial resistance to antibiotics of Beta lactam group is increasing worldwide threatening the public healthcare due to the limited treatment substitutions. Beta lactamase especially Extended Spectrum Beta Lactamase (ESBL) enzymes are responsible for the most important resistance mechanisms of the Enterobacteriacae to antibiotics of Beta lactam group. In this study, in order to investigate the prevalence of extended-spectrum β-lactamase among Enterobacteriacae isolated from clinically mastitis and apparently normal cattle. A total number of 500 milk samples were collected from cow milk. A total number of 100 ESBL Enterobacteriaceae isolates were obtained. Bacterial isolates were identified by VITEK 2 compact and then ESBL enzymes were identified phenotypically. Estimation of sensitivity of these isolates against variety of antibiotics by Disk Diffusion Sensitivity Test was carried out. Finally ESBL enzymes were identified by Real Time Polymerase Chain Reaction (rPCR). The results revealed that, out of the 100 Enterobacteriacae, 17 isolates (17 %) were positive phenotypically for ESBL. Of which, 14 isolates were positive for genotypically for ESBL. Furthermore, it was found that CTX-M Gene is the most prevalent gene in the Enterobacteriacae isolates

Key words: Enterobacteriacae,antibiotic resistance ,


 
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How to Cite this Article
Pubmed Style

Ibrahim ESF, wahab AMAE, Khalil SA, Torky HA. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. 2018; 58(1): 102-108. doi:10.5455/ajvs.285225

Web Style

Ibrahim ESF, wahab AMAE, Khalil SA, Torky HA. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. https://www.alexjvs.com/?mno=285225 [Access: December 02, 2024]. doi:10.5455/ajvs.285225

AMA (American Medical Association) Style

Ibrahim ESF, wahab AMAE, Khalil SA, Torky HA. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. 2018; 58(1): 102-108. doi:10.5455/ajvs.285225


Vancouver/ICMJE Style

Ibrahim ESF, wahab AMAE, Khalil SA, Torky HA. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. (2018), [cited December 02, 2024]; 58(1): 102-108. doi:10.5455/ajvs.285225


Harvard Style

Ibrahim, E. S. F., wahab, . A. M. A. E., Khalil, . S. A. & Torky, . H. A. (2018) Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS, 58 (1), 102-108. doi:10.5455/ajvs.285225


Turabian Style

Ibrahim, El Sayed F.H., Ashraf M. Abd El wahab, Samy A. Khalil, and Helmy A. Torky. 2018. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. Alexandria Journal of Veterinary Sciences, 58 (1), 102-108. doi:10.5455/ajvs.285225


Chicago Style

Ibrahim, El Sayed F.H., Ashraf M. Abd El wahab, Samy A. Khalil, and Helmy A. Torky. "Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk." Alexandria Journal of Veterinary Sciences 58 (2018), 102-108. doi:10.5455/ajvs.285225


MLA (The Modern Language Association) Style

Ibrahim, El Sayed F.H., Ashraf M. Abd El wahab, Samy A. Khalil, and Helmy A. Torky. "Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk." Alexandria Journal of Veterinary Sciences 58.1 (2018), 102-108. Print. doi:10.5455/ajvs.285225


APA (American Psychological Association) Style

Ibrahim, E. S. F., wahab, . A. M. A. E., Khalil, . S. A. & Torky, . H. A. (2018) Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. Alexandria Journal of Veterinary Sciences, 58 (1), 102-108. doi:10.5455/ajvs.285225




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    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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