E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 88
88Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo

Mohamed S.a. Elsayed, Heba Gamil S. Wahba, Shimaa S. Elnahriry.


Abstract
Escherichia coli (E. coli) is one of the most significant pathogens causing mastitis, one of the most complex and costly diseases in the dairy industry worldwide. This study aimed to isolate E. coli, detect virulence factors, assess antimicrobial susceptibility, and identify resistance genes in cows and buffaloes with clinical and subclinical mastitis.A total of 330 cows and 70 buffaloes were examined for clinical and subclinical mastitis. From cows, 50 clinical and 50 subclinical mastitis milk samples were collected, and from buffaloes, 30 clinical and 10 subclinical samples were collected. These samples were subjected to bacteriological identification, biochemical tests, antibiotic sensitivity testing, and molecular analysis.E. coli was isolated from 16/50 (32%) clinical and 5/50 (10%) subclinical mastitis samples from cows, and from 3/30 (10%) clinical and 1/10 (10%) subclinical samples from buffaloes. Antibiotic sensitivity testing revealed high resistance to oxytetracycline (48%), cefotaxime (24%), and streptomycin, gentamicin, and nalidixic acid (20%). Molecular analysis detected five virulence genes: fimA was the most prevalent gene, occurring in 83.3% of all isolates (clinical and subclinical). papC followed, present in 50% of isolates. The prevalence of iss and ompT was higher in subclinical isolates (66.6% for both) compared to clinical isolates (50% for iss and 44.4% for ompT). Conversely, iucD was slightly more common in clinical isolates (44.4%) than in subclinical ones (33.3%)..E. coli was identified as a major bacterial pathogen involved in bovine clinical and subclinical mastitis

Key words: E. coli, Mastitis, Virulence factors, Antimicrobial resistance


 
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How to Cite this Article
Pubmed Style

Elsayed MS, Wahba HGS, Elnahriry SS. Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. AJVS. 2026; 88(0): 72-80. doi:10.5455/ajvs.291421

Web Style

Elsayed MS, Wahba HGS, Elnahriry SS. Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. https://www.alexjvs.com/?mno=291421 [Access: February 04, 2026]. doi:10.5455/ajvs.291421

AMA (American Medical Association) Style

Elsayed MS, Wahba HGS, Elnahriry SS. Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. AJVS. 2026; 88(0): 72-80. doi:10.5455/ajvs.291421


Vancouver/ICMJE Style

Elsayed MS, Wahba HGS, Elnahriry SS. Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. AJVS. (2026), [cited February 04, 2026]; 88(0): 72-80. doi:10.5455/ajvs.291421


Harvard Style

Elsayed, M. S., Wahba, . H. G. S. & Elnahriry, . S. S. (2026) Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. AJVS, 88 (0), 72-80. doi:10.5455/ajvs.291421


Turabian Style

Elsayed, Mohamed S.a., Heba Gamil S. Wahba, and Shimaa S. Elnahriry. 2026. Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. Alexandria Journal of Veterinary Sciences, 88 (0), 72-80. doi:10.5455/ajvs.291421


Chicago Style

Elsayed, Mohamed S.a., Heba Gamil S. Wahba, and Shimaa S. Elnahriry. "Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo." Alexandria Journal of Veterinary Sciences 88 (2026), 72-80. doi:10.5455/ajvs.291421


MLA (The Modern Language Association) Style

Elsayed, Mohamed S.a., Heba Gamil S. Wahba, and Shimaa S. Elnahriry. "Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo." Alexandria Journal of Veterinary Sciences 88.0 (2026), 72-80. Print. doi:10.5455/ajvs.291421


APA (American Psychological Association) Style

Elsayed, M. S., Wahba, . H. G. S. & Elnahriry, . S. S. (2026) Bacteriological and Molecular Identification of E. coli Isolated from Clinical and Subclinical Mastitis in Cattle and Buffalo. Alexandria Journal of Veterinary Sciences, 88 (0), 72-80. doi:10.5455/ajvs.291421




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    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
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