E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
84Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim.


Abstract
Edwardsiella septicemia is a systemic bacterial disease caused by Edwardsiella tarda that is considered one of the most important bacterial microorganisms causing severe economic losses due to morbidity and mortality among wide fish species in many countries. In this study Edwardsiella tarda (E. tarda) was isolated and identified from Oreochromis niloticus fish farms using traditional and molecular PCR techniques using E. tarda specific primers. Fifty-six isolates of E. tarda were isolated from 300 fish samples during spring, summer and autumn. While in winter it couldn’t be isolated from 100 samples. The summer season showed the highest prevalence with 28% then the spring season with 18% finally autamn10%. Experimental infection using the isolated E. tarda was performed to determine the pathogenicity in O. niloticus through mortality rate, clinical sings, post mortem lesions and histopathological changes that were observed in the liver, spleen, kidney and gills of the infected fish. The result also showed that there is no significant difference between in vivo and in vitro effect of antibiotic on E. tarda. Norfloxacin showed the highest protection in vivo with 90% survival while Ampicillin showed no protection. These results indicated that the antibiotic of choice for treatment of E. tarda was Norfloxacin.

Key words: Edwardsiella tarda, Oreochromis niloticus, antibiotics, histopathology


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Eman Nagy
Articles by Amr Fadel
Articles by Fatma Abd Al-Moghny
Articles by Madiha S. Ibrahim
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. 2018; 57(1): 171-179. doi:10.5455/ajvs.294237

Web Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. https://www.alexjvs.com/?mno=294237 [Access: December 02, 2024]. doi:10.5455/ajvs.294237

AMA (American Medical Association) Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. 2018; 57(1): 171-179. doi:10.5455/ajvs.294237


Vancouver/ICMJE Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. (2018), [cited December 02, 2024]; 57(1): 171-179. doi:10.5455/ajvs.294237


Harvard Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim (2018) Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS, 57 (1), 171-179. doi:10.5455/ajvs.294237


Turabian Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. 2018. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. Alexandria Journal of Veterinary Sciences, 57 (1), 171-179. doi:10.5455/ajvs.294237


Chicago Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. "Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt." Alexandria Journal of Veterinary Sciences 57 (2018), 171-179. doi:10.5455/ajvs.294237


MLA (The Modern Language Association) Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim. "Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt." Alexandria Journal of Veterinary Sciences 57.1 (2018), 171-179. Print. doi:10.5455/ajvs.294237


APA (American Psychological Association) Style

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim (2018) Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. Alexandria Journal of Veterinary Sciences, 57 (1), 171-179. doi:10.5455/ajvs.294237




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use