Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress

Amal A. Halawa; Mohamed M. El-Adl; Basma H. Marghani

doi:10.5455/ajvs.297063

2025, Vol: 85

85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Original Article

AJVS. 2018; 57(2): 13-20

doi: 10.5455/ajvs.297063

Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress

Amal A. Halawa, Mohamed M. El-adl, Basma H. Marghani.

Abstract
The present work designed to estimate postmortem expression of mRNA of certain hepatic genes in rats in the presence or absence of postmortem heat stress using qRT-PCR technique in correlation to postmortem interval (PMI). Rats were sacrificed and divided into, control group (PMI=0 h), room temperature and heat stressed (41⁰C) groups. In control group, liver samples were collected immediately after death, while in the second and third groups, samples were collected at 1, 3 and 6 hours postmortem. The results showed significant reductions in pro-inflammatory gene transcripts (TNFα and IL-1β) at all-time points compared to control group with an inhibitory effect of postmortem heat stress on expression of TNFα at 1 and 6 h after death. Whereas postmortem expression of genes encode apoptosis was significantly increased at 3 and 6 h postmortem compared to control group. Postmortem heat stress reduced the hepatic expression of Bcl-2 and Caspase-3 at 3 and 6 h of PMI and increased the expression of Caspase-3 at 1 h after death compared to room temperature group. Meanwhile, postmortem hepatic mRNA expression of c-fos was significantly induced after death at 3 and 6 h PMI. Postmortem heat stress significantly reduced c-fos expression at 3 and 6 h after death. Thanatotranscriptome of genes encode inflammation, apoptosis as well as c-fos varied in their correlations to PMI. Additionally, the results showed disparities in the effect of postmortem heat stress according to the studied gene and time.

Key words: Thanatotranscriptome, postmortem, heat stress, apoptosis, inflammation

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Amal A. Halawa Articles by Mohamed M. El-Adl Articles by Basma H. Marghani
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Halawa AA, El-adl MM, Marghani BH. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. 2018; 57(2): 13-20. doi:10.5455/ajvs.297063

Web Style

Halawa AA, El-adl MM, Marghani BH. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. https://www.alexjvs.com/?mno=297063 [Access: March 25, 2025]. doi:10.5455/ajvs.297063

AMA (American Medical Association) Style

Halawa AA, El-adl MM, Marghani BH. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. 2018; 57(2): 13-20. doi:10.5455/ajvs.297063

Vancouver/ICMJE Style

Halawa AA, El-adl MM, Marghani BH. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. (2018), [cited March 25, 2025]; 57(2): 13-20. doi:10.5455/ajvs.297063

Harvard Style

Halawa, A. A., El-adl, . M. M. & Marghani, . B. H. (2018) Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS, 57 (2), 13-20. doi:10.5455/ajvs.297063

Turabian Style

Halawa, Amal A., Mohamed M. El-adl, and Basma H. Marghani. 2018. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. Alexandria Journal of Veterinary Sciences, 57 (2), 13-20. doi:10.5455/ajvs.297063

Chicago Style

Halawa, Amal A., Mohamed M. El-adl, and Basma H. Marghani. "Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress." Alexandria Journal of Veterinary Sciences 57 (2018), 13-20. doi:10.5455/ajvs.297063

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article