E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 91
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Original Article
Online Published: 23 Sep 2026
 


Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle

Marina Aziz, Olla A. Khalifa, Samar H. Baloza, Amany Abbass, Fatma El-gendy.


Abstract
Background: The bovine fatty acid synthase (FASN) gene encodes a multifunctional enzyme that carries out de novo fatty acid synthesis in the mammary gland. Its main product is palmitic acid (C16:0), and this activity helps shape the milk fat profile. However, information regarding the association of FASN polymorphisms with milk production traits in Egyptian Holstein–Friesian cattle remains limited.
Method: Samples of blood and milk were obtained from 70 Holstein-Friesian dairy cattle. DNA was extracted from blood samples for the PCR amplification of a 353 bp fragment of the FASN gene. The HhaI restriction enzyme was employed for RFLP investigations focusing on SNP g.17924A>G located in exon 34 of FASN gene, followed by direct DNA sequencing. The composition of milk was analyzed by Lactoscan milk analyzer.
Results: RFLP investigation revealed polymorphism with three genotypes: AA, AG, and GG. The GG genotype exhibited a significant increase in milk yield, fat, protein, and lactose percentages. Direct DNA sequencing confirmed the g.17924A>G non-synonymous SNP that causes a threonine-to-alanine amino acid substitution, in addition to a C/T synonymous SNP.
Conclusion: The current finding indicates that genetic polymorphism in the FASN gene was associated with milk yield and composition traits in Holstein-Friesian dairy cows. The favorable performance of the GG genotype suggests that the g.17924A>G polymorphism could serve as a promising candidate marker for incorporation into marker-assisted selection (MAS) strategies to improve milk yield and composition traits and overall dairy breeding efficiency.

Key words: FASN polymorphism, Holstein-Friesian cows, PCR-RFLP, sequencing, milk composition.


 
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How to Cite this Article
Pubmed Style

Aziz M, Khalifa OA, Baloza SH, Abbass A, El-gendy F. Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. AJVS. 2026; 91(0): 288-296. doi:10.5455/ajvs.325952

Web Style

Aziz M, Khalifa OA, Baloza SH, Abbass A, El-gendy F. Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. https://www.alexjvs.com/?mno=325952 [Access: October 18, 2026]. doi:10.5455/ajvs.325952

AMA (American Medical Association) Style

Aziz M, Khalifa OA, Baloza SH, Abbass A, El-gendy F. Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. AJVS. 2026; 91(0): 288-296. doi:10.5455/ajvs.325952


Vancouver/ICMJE Style

Aziz M, Khalifa OA, Baloza SH, Abbass A, El-gendy F. Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. AJVS. (2026), [cited October 18, 2026]; 91(0): 288-296. doi:10.5455/ajvs.325952


Harvard Style

Aziz, M., Khalifa, . O. A., Baloza, . S. H., Abbass, . A. & El-gendy, . F. (2026) Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. AJVS, 91 (0), 288-296. doi:10.5455/ajvs.325952


Turabian Style

Aziz, Marina, Olla A. Khalifa, Samar H. Baloza, Amany Abbass, and Fatma El-gendy. 2026. Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. Alexandria Journal of Veterinary Sciences, 91 (0), 288-296. doi:10.5455/ajvs.325952


Chicago Style

Aziz, Marina, Olla A. Khalifa, Samar H. Baloza, Amany Abbass, and Fatma El-gendy. "Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle." Alexandria Journal of Veterinary Sciences 91 (2026), 288-296. doi:10.5455/ajvs.325952


MLA (The Modern Language Association) Style

Aziz, Marina, Olla A. Khalifa, Samar H. Baloza, Amany Abbass, and Fatma El-gendy. "Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle." Alexandria Journal of Veterinary Sciences 91.0 (2026), 288-296. Print. doi:10.5455/ajvs.325952


APA (American Psychological Association) Style

Aziz, M., Khalifa, . O. A., Baloza, . S. H., Abbass, . A. & El-gendy, . F. (2026) Association of Fatty Acid Synthase (FASN) Gene Polymorphisms with Milk Yield and Composition Traits in Egyptian Holstein-Friesian Cattle. Alexandria Journal of Veterinary Sciences, 91 (0), 288-296. doi:10.5455/ajvs.325952




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Abees 10th district, Alexandria
    Egypt.

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