E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 91
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Original Article
Online Published: 03 Oct 2026
 


The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.

Shaimaa M. Tony, Ahmed M. Mostafa, Mona M. Abd El- Aziz, Huda Elsayed, Elsaid M. Saafan, Azza M. Abdelmoteleb, Ashraf A. Hamoda.


Abstract
Histamine is a type of biogenic amine. It poses significant toxicological risks in long-storage dairy products such as Ras cheese. This study evaluated the efficacy of Nigella sativa oil (NSO) at varying concentrations (0.1%, 0.3%, and 0.5%) during a 90-day refrigerated storage period. Values of pH and salt percentages showed no significant differences among the treatments at zero time. After 60 and 90 days, the 0.5% treatment recorded significantly lower pH values compared to the control and 0.1% levels, while salt percentages stabilized across all groups without significant variations. For protein degradation (TVB-N), the control samples exhibited the highest TVB-N levels throughout the entire storage period, whereas the 0.3% and 0.5% concentrations significantly reduced these values (p<0.05). Regarding histamine content, the 0.5% concentration yielded significantly lower histamine levels compared to the control and all other treatments across the storage periods (p<0.05). In terms of sensory evaluation, the 0.1% treatment achieved the highest taste acceptability scores by Day 90. While the 0.3% and 0.5% concentrations initially received lower sensory scores for taste and odor, the extended storage period may have allowed the oil components to gradually integrate well into the cheese matrix, resulting in equalized and stable scores. Regarding consistency, scores stabilized across all treatments from Day 30 to Day 90, showing no significant differences. In conclusion, Nigella sativa oil showed potential in mitigating histamine accumulation. These findings suggest its application as a promising natural additive to delay quality deterioration while maintaining the physicochemical and sensory quality of hard cheeses.

Key words: Histamine, Ras cheese, Nigella sativa, Biogenic amines, Natural additives.


 
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How to Cite this Article
Pubmed Style

Tony SM, Mostafa AM, Aziz MMAE, Elsayed H, Saafan EM, Abdelmoteleb AM, Hamoda AA. The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. AJVS. 2026; 91(0): 382-391. doi:10.5455/ajvs.328736

Web Style

Tony SM, Mostafa AM, Aziz MMAE, Elsayed H, Saafan EM, Abdelmoteleb AM, Hamoda AA. The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. https://www.alexjvs.com/?mno=328736 [Access: October 18, 2026]. doi:10.5455/ajvs.328736

AMA (American Medical Association) Style

Tony SM, Mostafa AM, Aziz MMAE, Elsayed H, Saafan EM, Abdelmoteleb AM, Hamoda AA. The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. AJVS. 2026; 91(0): 382-391. doi:10.5455/ajvs.328736


Vancouver/ICMJE Style

Tony SM, Mostafa AM, Aziz MMAE, Elsayed H, Saafan EM, Abdelmoteleb AM, Hamoda AA. The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. AJVS. (2026), [cited October 18, 2026]; 91(0): 382-391. doi:10.5455/ajvs.328736


Harvard Style

Tony, S. M., Mostafa, . A. M., Aziz, . M. M. A. E., Elsayed, . H., Saafan, . E. M., Abdelmoteleb, . A. M. & Hamoda, . A. A. (2026) The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. AJVS, 91 (0), 382-391. doi:10.5455/ajvs.328736


Turabian Style

Tony, Shaimaa M., Ahmed M. Mostafa, Mona M. Abd El- Aziz, Huda Elsayed, Elsaid M. Saafan, Azza M. Abdelmoteleb, and Ashraf A. Hamoda. 2026. The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. Alexandria Journal of Veterinary Sciences, 91 (0), 382-391. doi:10.5455/ajvs.328736


Chicago Style

Tony, Shaimaa M., Ahmed M. Mostafa, Mona M. Abd El- Aziz, Huda Elsayed, Elsaid M. Saafan, Azza M. Abdelmoteleb, and Ashraf A. Hamoda. "The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.." Alexandria Journal of Veterinary Sciences 91 (2026), 382-391. doi:10.5455/ajvs.328736


MLA (The Modern Language Association) Style

Tony, Shaimaa M., Ahmed M. Mostafa, Mona M. Abd El- Aziz, Huda Elsayed, Elsaid M. Saafan, Azza M. Abdelmoteleb, and Ashraf A. Hamoda. "The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.." Alexandria Journal of Veterinary Sciences 91.0 (2026), 382-391. Print. doi:10.5455/ajvs.328736


APA (American Psychological Association) Style

Tony, S. M., Mostafa, . A. M., Aziz, . M. M. A. E., Elsayed, . H., Saafan, . E. M., Abdelmoteleb, . A. M. & Hamoda, . A. A. (2026) The Influence Of Adding Nigella Sativa Oil Extract On Histamine Levels In Ras Cheese During Storage.. Alexandria Journal of Veterinary Sciences, 91 (0), 382-391. doi:10.5455/ajvs.328736




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    Abees 10th district, Alexandria
    Egypt.

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