E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek.

Cited By: 2

Abstract
Seventeen avian infectious bronchitis virus (IBV) isolates were isolated from broiler chickens showing respiratory and renal lesions. The isolated strains were characterized by real time reverse transcriptase polymerase chain reaction used for N gene, and then RT-PCR and sequence analysis of the hypervariable region 3 of the S1 spike glycoprotein gene of six isolates. Six isolates showed 87.15% to 89.71 % and 87.27% to 90.82% amino acid sequence identity and 87.61% to 89.19 % and 87.91% to 89.72% nucleotide sequence identity to the Egyptian variant 1 and the IS/885 strains, respectively. The six isolates formed a distinct phylogenetic group with the Ck/Eg/BSU-2/2011 and Ck/Eg/ BSU-3/2011 (Var 2). Amino acid and nucleotide identities between the six Egyptian isolates and variant 2 (Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011) ranged from 97.27% to 100% and 97.88% to 99.38%, respectively. The results indicate that the six isolates IBV/CK/Beh/101/013/S1,IBV/CK/Beh/204/013/S1,IBV/CK/Beh/105/013/S1,IBV/CK/Beh/1011/013/S1,IBV/CK/Beh/1017/013/S1,IBV/CK/Beh/2020/013/S1 can be considered a variant 2 as Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011. This study demonstrates a constant evolution of IBV in Egypt that necessitates continuous monitoring to control the spread of infections, and the development and use of vaccines based on indigenous viruses.

Key words: Antigenic variations, Infectious Bronchitis virus, RT-PCR, Sequencing.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sarah E. AlBeltagi
Articles by Helmy A. Torky
Articles by Mahmoud E. Seddeek
on Google
on Google Scholar


REFERENCES
Abdel-Moneim AS, El-Kady MF, Ladman BS, Gelb J Jr. 2006. S1 gene sequence analysis of a nephro-pathogenic strain of avian infectious bronchitis virus in Egypt. Virology J, 3:78. [DOI via Crossref]   
Abdel-moneim, A.S., Madbouly, H.M., Gelb, J.R. and Ladman, B.S. 2002. Isolation and identification of Egypt/Beni-Suef/01 a novel genotype of infectious bronchitis virus. Vet Med J Giza, 50: 1065-1078.
Abdel-moneim, A.S., Afifi, M.A., El-Kady, M.F. 2012. Emergence of a novel genotype of avian infectious bronchitis virus in Egypt. Arch Virol, 157:2453–2457.
Ahmed HN. 1954. Incidence and treatment of some infectious viral respiratory diseases of poultry in Egypt. PhD thesis Fac Vet Med Cairo Univ Egypt.
Antarasena, C., Nuansrichay, B., Thongmee, S. and Intarasarn, M. 2008. S1 Glycoprotein Gene Analysis of Infectious Bronchitis Virus Strains Isolated in Southern Thailand Proceedings, The 15th Congress of FAVA - OIE Joint Symposium on Emerging Diseases (pp 147-150), Bangkok, Thailand.
Boursnell, M.E.G., Brown, T.D.K., Foulds, I.J. Green, P.F., Tomley, F.M., Binns, M.M. 1987. Completion of the sequence of the genome of coronavirus avian infectious bronchitis virus. J. Gen. Virol. 68, 55-77. [DOI via Crossref]   
Cavanagh, D. 1981. Structural polypeptides of coronavirus IBV. J. Gen. Virol. 53:93. [DOI via Crossref]   
Cavanagh, D. 2007. Coronavirus avian infectious bronchitis virus. Vet. Res, 38:281- 297. [DOI via Crossref]   
Cavanagh, D. 1984. Structural characterization of IBV glycoproteins. Adv. Exp. Med. Biol. 173:95. [DOI via Crossref]   
Cavanagh, D. 1983. Coronavirus IBV: structural characterization of the spike protein. J. Gen. Virol. 64: 2577-2583. [DOI via Crossref]   
Cavanagh, D. 1997. Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 142: 629-633.
Cavanagh, D. 1995. The coronavirus surface glycoprotein. In: The Coronaviridae. S. G. Siddel, ed. Plenum press, New York. pp 73- 103. [DOI via Crossref]   
Cavanagh, D. and Naqi, S. 2003. Infectious bronchitis, In diseases of poultry, Y. M. SAIF, H. J. BARNES, J. R. GLISSON, A.M. FADLY, L.R. McDOUGALD, and D.E. Swayne, eds. (Ames, Iowa: Iowa state university Press), pp 101-119.
Cavanagh, D., and P. J. Davis. 1986. Coronavirus IBV: removal of spike glycopolypeptide S1 by urea abolishes infectivity and haemagglutination but not attachment to cells. J. Gen. Virol. 67:1443. [DOI via Crossref]   
Cavanagh, D., Davis, P., Cook, J., Li, D., 1990. Molecular basis of the variation exhibited by avian infectious bronchitis coronavirus (IBV). Adv. Exp. Med. Biol. 276, 369–372.
Cavanagh D, Davis PJ, Cook J. 1992. Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathol 21:401–408.
Cavanagh D, Davis PJ, Mockett APA. 1988. Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes. Virus Res 11:141–150.
Chubb, R.C. and Cumming,R.B. 1972. The use of the gel diffusion precipitin technique with avian infectious bronchitis Nephritis viruses. Aust. Vet. Jou, 47: 496-499. [DOI via Crossref]   
El-Kady MF. 1989. Studies on the epidemiology and means of control of infectious bronchitis disease in chickens in Egypt, PhD thesis. Faculty of Veterinary Medicine, Cairo University, Egypt.
Gelb, J., Jr. and Jackwood, M.K. 1998. Infectious bronchitis. In: A laboratory manual for the Isolation and Identification of Avian Pathogens. 4th Ed. AAAP: 169-174.
Gelb J. Jr., Weisman Y., Ladman B.S., Meir R. 2005. S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000), Avian Pathol. 34:194–203.
Gorgyo, M., Umemura, T. and Itakura, C. 1984. Concurrence of nephrosis- nephritis due to infectious bronchitis virus and infectious bursal disease in broiler chickens. Avian Pathology, 13: 191-200. [DOI via Crossref]   
Holmes KV, Lai MM. 2001. Coronaviridae: the virus and their replication. In: Fields BN, Knipe DM, Howley PM (eds) Fundamental virology, 4th edn. Lippincott-Raven, Philadelphia, pp 1163–1203.
Jackwood MW, Hall D, Handel A. 2012. Molecular evolution and emergence of avian gammacoronaviruses. Infect Genet Evol 12(6):1305–1311.
Jackwood, M.W., Yousef, N.M., Hilt, D.A. 1997. Further development and use of a molecular serotype identification test for infectious bronchitis virus. Avian Dis. 41, 105–110.
Kant, A., G. Koch, D. J. Van Roozelaar, J. G. Kusters, F. A. Poelwijk, and B. A. van der Zeijst. 1992. Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. J. Gen. Virol. 73:591. [DOI via Crossref]   
Koch, G., L. Hartog, A. Kant, and D. J. Van Roozelaar. 1990. Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. J. Gen. Virol. 71:1929. [DOI via Crossref]   
Kwon, H.M., Jackwood, M.W. & Gelb, J. 1993. Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Diseases, 37, 194– 202.
Lee, C.W., Hilt, D.A., Jackwood, M.W. 2000. Redesign of primer and application of the reverse transcriptase-polymerase chain reaction and restriction fragment length polymorphism test to the DE072 strain of infectious bronchitis virus. Avian Dis. 44, 650–654.
Lin, Z., Kato, A., Kudou, Y. & Ueda, S. 1991. A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism. Archives of Virology, 116, 19– 31.
Meir, R., Maharat, O., Farnushi, Y. and Simanov, L. 2010. Development of a real- time TaqMan® RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation. Journal of Virological Methods, 163: 190–194.
Mockett, A.P.A., Cavanagh, D. & Brown, T.D.K. 1984. Monoclonal antibodies to the S1 spike and membrane proteins of avain infectious bronchitis virus coronavirus strain Massachusetts M41. Journal of General Virology, 65, 2281–2286.
Moore KM, Jackwood MW, Hilt DA. 1997. Identification of amino acids involved in a serotype and neutralization specific epitope within the S1 subunit of avian infectious bronchitis virus. Arch Virol 142:2249–2256.
Schalk, A.F. and Hawn, M.C. 1931. An apparently new respiratory disease of baby chicks. J. Am. Vet. Med. Assoc. 78: 413- 422.
Sheble, A., Sabry, M.Z., Davelaar, F.G., Burger, A.G., Khafagy, A.K., Moustafa, F., Moustafa, M.M. and Henna, M. 1986. Present status of infectious bronchitis in Egypt. J Egyp Vet Med Assoc. 4: 393-411.
Stern DF, Sefton BM. 1982. Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. J. Virol 44:804–812.
Spaan, W., Cavanagh, D., Horzinek, M.C., 1988. Coronaviruses: structure and genome expression. J. Gen. Virol. 69, 2939-2952. [DOI via Crossref]   
Sultan, H.A., Tantawi, L., Youseif, A.I. and Ahmed, A.A.S. 2004. Urolithiathis in white commercial egg laying chickens associated with an infectious bronchitis virus. 6th Sci Conf Egyp Vet Poult Assoc 155-169.
Sutou, S., Sato, S., Okabe, T., Nakai, M., Sasaki, N., 1988. Cloning and sequencing of genes encoding structrural proteins of avian infectious bronchitis virus, Virology 165, 589-595. [DOI via Crossref]   

This Article Cited By the following articles

Using T Cell Lymphokines of Hyperimmunized Chickens with Salmonella enteritidis to Protect Neonatal Broiler Chicks Against Infectious Bronchitis Disease
International J. of Poultry Science 2018; 17(10): 493.

1
 
Infectious Bronchitis Virus Genotypes in the Middle East
Avian Diseases 2021; 65(4): .

2
 
How to Cite this Article
Pubmed Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652


Web Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. https://www.alexjvs.com/?mno=46652 [Access: April 29, 2023]. doi:10.5455/ajvs.46652


AMA (American Medical Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652



Vancouver/ICMJE Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. (2014), [cited April 29, 2023]; 40(1): 44-51. doi:10.5455/ajvs.46652



Harvard Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS, 40 (1), 44-51. doi:10.5455/ajvs.46652



Turabian Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. 2014. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652



Chicago Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40 (2014), 44-51. doi:10.5455/ajvs.46652



MLA (The Modern Language Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40.1 (2014), 44-51. Print. doi:10.5455/ajvs.46652



APA (American Psychological Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 16 times [Click to see citing articles]

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 12 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 9 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 9 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 7 times [Click to see citing articles]