Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis

Eman S. Ramadan; Amal H. Ali; Lamees A. El-Tantawy; Ibrahim G. Ibrahim

doi:10.5455/ajvs.47618

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Original Article

AJVS. 2019; 61(2): 32-37

doi: 10.5455/ajvs.47618

Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis

Eman S. Ramadan, Amal H. Ali, Lamees A. El-Tantawy, Ibrahim G. Ibrahim.

Abstract
To address the effect of serum treatment with chloroform (STC) on increasing the specificity of the Rose Bengal test (RBT), 200 serum samples (n=100 buffaloes and n=100 cows) were tested. For each animal species, 50 were apparently healthy and 50 were aborted. RBT and indirect enzyme-linked immunosorbent assay (iELISA) were applied for testing the serum before and after treatment with chloroform. The coherence between tests was poor (K=0.004, SE=0,003), (K=0.031, SE=0.24) and (K=0.176, SE=0,143) for apparently healthy buffaloes, apparently healthy cows and aborted buffaloes respectively, but it was only fair (K=0.386, SE=0,185) for aborted cows. After STC, the coherence between tests was markedly improved, where it became perfect (K=1.0, SE=0.0), (K=0.834, SE=0.113) and very good (K=0.896, SE=0.071) for apparently healthy buffaloes and cows, aborted cows and aborted buffaloes. The specificity and sensitivity of RBT were obviously raised after STC recording (100% and 100%), (80% and 100%) and (75% and 100%) for apparently healthy buffaloes and cows, aborted buffaloes and aborted cows respectively. Current results recommended that STC is a reliable method to avoid false positives. The higher specificity and sensitivity obtained by RBT after using STC suggests that it is not only can be used as a rapid, simple, economical and convenient screening method but also it can be used as a confirmatory test for serodiagnosis of brucellosis for large scale in endemic areas.

Key words: Brucellosis, Chloroform, RBT, Serum treatment, Specificity.

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Pubmed Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. 2019; 61(2): 32-37. doi:10.5455/ajvs.47618

Web Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. https://www.alexjvs.com/?mno=47618 [Access: December 02, 2024]. doi:10.5455/ajvs.47618

AMA (American Medical Association) Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. 2019; 61(2): 32-37. doi:10.5455/ajvs.47618

Vancouver/ICMJE Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. (2019), [cited December 02, 2024]; 61(2): 32-37. doi:10.5455/ajvs.47618

Harvard Style

Ramadan, E. S., Ali, . A. H., El-Tantawy, . L. A. & Ibrahim, . I. G. (2019) Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS, 61 (2), 32-37. doi:10.5455/ajvs.47618

Turabian Style

Ramadan, Eman S., Amal H. Ali, Lamees A. El-Tantawy, and Ibrahim G. Ibrahim. 2019. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. Alexandria Journal of Veterinary Sciences, 61 (2), 32-37. doi:10.5455/ajvs.47618

Chicago Style

Ramadan, Eman S., Amal H. Ali, Lamees A. El-Tantawy, and Ibrahim G. Ibrahim. "Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis." Alexandria Journal of Veterinary Sciences 61 (2019), 32-37. doi:10.5455/ajvs.47618

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