E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly.

Abstract
A total of 257 serum samples including, equine (56), birds (95) and human (106) were collected from rural area in Behera Province during the period extended from the beginning of June to the end of October 2013 to be examined for presence of antibodies against West Nile virus by ELISA. In addition, a total of 943 mosquitoes (25 mosquitoes groups) were collected by CDC light trap from the same place and during the same period where homogenized tissue samples of mosquitoes were obtained to be examined for presence of viral RNA of WNV by real time RT-PCR. The serological examination of serum samples clarified that the prevalence of IgG antibodies against WNV was 100, 4.2 and 58.5 % in equine, birds and human serum samples, respectively. Moreover, some epidemiological aspects of human samples were studied including gender, age and health status. Statistical analysis indicated significant differences in detection of IgG antibodies against WNFV in different genders where the higher detection rate was observed in females (42.30 %), than in males (35 %), in different age groups where the higher detection rate was observed in age group 40 - < 60 years (83.33 %) followed by age group 20 - <40 years (42.85 %), then the age group 60 - < 80 (37.50%) and the least detection rate of IgG was observed in age group 1 - < 20 years (16.66 %) and in health status where the highest detection rate was recorded in meningitis patients (80.9%) followed by FUO patients (64.4%) and lastly apparent healthy individuals (40%). On the other side, real time RT-PCR examination of samples of mosquitoes clarified no detection of the viral RNA of WNV in all examined samples. The obtained results confirmed the endimicity of WNV in equine in Egypt and the role played by birds in transmission of WNV to human. Although, examined mosquitoes were found to negative for presence of the viral RNA, their role in transmission of WNV could not denied.

Key words: WNF, Animals, Human, Mosquitoes, Serology, RT- PCR


 
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REFERENCES
Allison, A. B., Mead, D. G., Gibbs, S. E., Hoffman, D. M., Stallknecht, D. M. 2004. West Nile Virus Viremia in Wild Rock Pigeons. J. Emerg. Infect. Dis. (10): 2252-2255.
Bahri, O., Dhifallah, I., Ben, Alaya-Bouafif, N., Fekih, H., Gargouri, J., Triki, H. 2011. Sero- epidemiological study of West Nile virus circulation in human in Tunisia. Bull Soc Pathol Exot., 104 (4): 272-276 [DOI via Crossref]   
Blackmore, C. G. M., Stark, L. M., Jeter, W. C., Oliveri, R. L., Brooks, R. G., Comti, L. A., Wiersma, S. T. 2003. Surveillance results from the 1st West Nile Virus transmission season in Florida, 2000. Am. J. Top. Med. Hyg. (69): 141-150.
Calzolari, M., Bonilauri, P., Bellini, R., Albieri, A., Defilippo, F., Maioli, G., Galletti, G., Gelati, A., Barbieri, I., Tamba, M., Lelli, D., Carra, E., Cordioli, P., Angelini, P., Dottor, M. 2010. Evidence of Simultaneous Circulation of West Nile and Usutu Viruses in Mosquitoes Sampled in Emilia Romagna Region (Italy) in 2009. J. PLoS ONE 5 (12): e14324. [DOI via Crossref]   
Carson, P. J., Borchardt, S. M., Custer, B., Prince, H. E., Dunn-Williams, J., Winkelman, V., Tobler, L., Biggerstaff, B. J., Lanciotti, R., Petersen, L. R., Busch, M. P. 2012. Neuroinvasive disease and West Nile virus infection, North Dakota, USA, 1999-2008. J. Emerg Infect Dis., 18(4): 684-686. [DOI via Crossref]   
Castillo-Olivares, J. and Wood, J. 2004. West Nile virus infection of horses. Vet. Res. (35): 467-483. Chinikar, S., Shah-Hosseini, N., Mostafavi, E., Moradi, M., Khakifirouz, S., Jalali, T., Goya, M.M., Shirzadi, M.R., Zainali, M. and Fooks, A.R. 2013. Seroprevalence of West Nile Virus in Iran. Vector. Borne. Zoonotic Dis. Epub ahead of print. El Rhaffouli, H., El Harrak, M., Lotfi, C., El Boukhrissi, F., Bajjou, T., Laraqui, A, Hilali, F., Kenfaoui, M. and Lahlou-Amine, I. 2012. Serologic evidence of West Nile virus infection among humans, Morocco. J. Emerg. Infect. Dis. 18 (5): 880-881.
Franson, J. C., Hofmeister, E. K., Collins, G. H., Dusek, R.G. 2011. Short Report: Seroprevalence of West Nile Virus in Feral Horses on Sheldon National Wildlife Refuge, Nevada, United States. Am. J. Trop. Med. Hyg. 84(4): 637-640. [DOI via Crossref]   
Garcia-Bocanegra, I., Arenas-Montes, A., Jaen- Tellez, J. A., Napp, S., Fernandez-Morente, M., Arenas, A. 2012. Use of sentinel serosurveillance of mules and donkeys in the monitoring of West Nile Virus infection. J. Vet. (194): 262-264
Gibbs, S. E., Allison,A. B., Yabsley, M. J., Mead, D. G., Wilcox, B. R., Stallknecht, D. E. 2006 West Nile virus antibodies in avian species of Georgia, USA: 2000-2004. J. Vector borne Zoonotic. Dis. (6): 57-72. [DOI via Crossref]   
Hayes, E. B., Komar, N., Nasci, R. S., Montgomery, S. P., O'Leary, D. R., Campbell, G. L. 2005.Epidemiology and transmission dynamics of West Nile virus disease. J. Emerg. Infect. Dis. 11(8): 1167-1173. [DOI via Crossref]   
Hubalek, Z., Kriz, B., Halouzka, J. 2011. Serologic survey of humans for flavivirus West Nile in Southern Moravia (Czech Republic). Cent. Eur. J. Public. Health. 19 (3): 131-133.
Jourdain, E., Zeller, H. G., Sabatie, P., Murri, S., Kayse, Y., Greenland, T., Lafaye, M., Gauthier- Clerc, M. 2008. Prevalence of West Nile Virus Neutralizing Antibodies in Wild Birds from the Camargue Area, Southern France. J. Wildlife Diseases (JWD). (44): 766-771. [DOI via Crossref]   
Kalaycioglu, H., Korukluoglu, G., Ozkul, A., Oncul, O., Tosun, S., Karabay, O., Gozalan, A., Uyar, Y., Caglayik, D. Y., Atasoylu, G., Altas, A. B., Yolbakan, S., Ozden, T. N., Bayrakdar, F., Sezak, N., Pelitli, T. S., Kurtcebe, Z. O., Aydin, E., Ertek, M. 2012. Emergence of West Nile virus infections in humans in Turkey, 2010 to 2011. J. Euro Surveill. 24, 17(21).
Kleiboeker, S. B., Loiacono, C. M., Rottinghaus, A., Pue, H. L., Johnson, C. G. 2004. Diagnosis of West Nile virus infection in horses. J. Vet. Diag. Invest. (16): 2-10. [DOI via Crossref]   
Kubica-Biernat, B., Kruminis-Lozowska, W., Stanczak, J. and Cieniuch, S. 2009. A study on the occurrence of West Nile virus in mosquitoes (Dipetra: Culicidae) on the selected areas in Poland. J. Wiad Parazytol. 55(3):259- 263.
Kuwahara, M., Kitai, Y., Kondo, T., Konishi, E. 2012. Survey on Antibodies Specific for West Nile Virus in Horses from 2006 to 2010 in Japan. Jpn. J. Infec. Dis. (65): 553-555.
Ladbury, G. A., Gavana, M., Danis, K., Papa, A., Papamichail, D., Mourelatos, S., Gewehr, S., Theocharopoulos, G., Bonovas, S., Benos, A., Panagiotopoulos, T. 2013. Population seroprevalence study after a West Nile virus lineage 2 epidemic, Greece, 2010. J. PLoS One. 18, 8 (11).
Maeda, A. and Meada, J. 2013. Review of diagnostic plaque reduction neutralization tests for flavivirus infection. J. Vet. (195): 33-40.
Mattingly, P. F. 1971. Contributions to the mosquito fauna of Southeast Asia. XGG. Illustrated keys to the genera of mosquitoes (Diptera; Culicidae). Contributions of American Entomological Institute, (7): 1- 48.
Megan, J. M., Khalil, G. M., Hoogstraal, H., Adham, F.K. 1980. Experimental transmission and field isolation studies implicating Culex pipiens as a vector of Rift Valley fever virus in Egypt. Am. J. Trop. Med. Hyg. 29(6): 1405-1410. Mostashari, F., Bunning, M. L., Kitsutani, P. T., Singer, D. A., Nash, D., Cooper, M. J, Katz, N., Liljebjelke, K. A., Biggerstaff, B. J., Fine, A. D., Layton, M. C., Mullin, S. M., Johnson, A.
J., Martin, D. A., Hayes, E. B., Campbell, G. L. 2001. Epidemic West Nile encephalitis, New York, 1999: results of a household-based seroepidemiological survey. Lancet. (358): 261- 264.
Nash, D., Mostashari, F., Fine, A., Miller, J., O'Leary, D., Murray, K., Huang, A., Rosenberg, A., Greenberg, A., Sherman, M., Wong, S. and Layton, M. 2001. The outbreak of West Nile virus infection in the New York City area in 1999. N. Engl. J. Med. (344): 1807–1814. [DOI via Crossref]   
Ostlund, E. N., Crom, R. L., Pedersen, D. D., Johnson, D. J., Williams, W. O., Schmitt, B. J. 2011. Equine West Nile Encephalitis, United States. Emerg. Infect. Dis. (7): 665-669.
Petersen, L. R. and Roehrig, J. T. 2001. West Nile Virus: A reemerging global pathogen. Emerg. Infect. Dis. 7(4): 611-614. [DOI via Crossref]   
Reiter, P. 1983. A portable battery powered trap for collecting gravid Culex mosquitoes". Mosquitoes News. (43): 496-498.
Romich, J. A. 2008. Understanding zoonotic diseases. 2nd ed. Library of congress Canada.
Smithburn, K. C., Hughes, T. P., Burke, A.W., Paul, J. H. 1940. A neurotropic virus isolated from the blood of a native of Uganda. Am. J. Trop. Med. Hyg. (20): 470-492.
Soliman, A., Mohareb, E., Salman, D., Saad, M., Salama,S., Fayez, C., Hanafi, H., Medhat, I., Labib, E., Rakha, M., El-Sayed, N., Yingst,S., Tjaden, J., Eahart, K. 2010. Studies on west Nile virus infection in Egypt. J. Infect. Public Health. (3): 54-59.
Spigland, I., Jasinska-Klingberg, W., Hofshi, E. Goldblum, N. 1958. Clinical and laboratory observations in an outbreak of West Nile fever in Israel in 1957. J. Harefuah. (54): 275- 281.

How to Cite this Article
Pubmed Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. AJVS. 2014; 40(1): 77-86. doi:10.5455/ajvs.49148


Web Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. https://www.alexjvs.com/?mno=49148 [Access: May 04, 2023]. doi:10.5455/ajvs.49148


AMA (American Medical Association) Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. AJVS. 2014; 40(1): 77-86. doi:10.5455/ajvs.49148



Vancouver/ICMJE Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. AJVS. (2014), [cited May 04, 2023]; 40(1): 77-86. doi:10.5455/ajvs.49148



Harvard Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly (2014) Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. AJVS, 40 (1), 77-86. doi:10.5455/ajvs.49148



Turabian Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. 2014. Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. Alexandria Journal of Veterinary Sciences, 40 (1), 77-86. doi:10.5455/ajvs.49148



Chicago Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. "Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province." Alexandria Journal of Veterinary Sciences 40 (2014), 77-86. doi:10.5455/ajvs.49148



MLA (The Modern Language Association) Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly. "Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province." Alexandria Journal of Veterinary Sciences 40.1 (2014), 77-86. Print. doi:10.5455/ajvs.49148



APA (American Psychological Association) Style

Hamed A. Samaha, Yaser N. Haggag, Mohammad A. Nossair, Haitham F. Adly (2014) Occurrence of West Nile fever in Equine, Birds, Human and Mosquitoes in Behera Province. Alexandria Journal of Veterinary Sciences, 40 (1), 77-86. doi:10.5455/ajvs.49148





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