E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver

Mohamed M. Elsenduony, Wafaa M. Bakr, Abd El Rahman M. Wafa, Eman A. Omran.

Abstract
Escherichia coli is known as one of the most important foodborne pathogens in humans. Contaminated chicken meat is an important source of foodborne infection with this bacterium. A total of 144 fresh chicken meat samples (72 from chicken breasts and 72 from chicken thighs) were collected from different six farms so that 24 samples were collected from each farm. One of the farms was considered as a control, as its poultry did not receive any antibiotics as food promoters, while chickens in the remaining five farms received antibiotics (Doxycycline 50% and Tylosin 20%). In addition, 72 chicken liver samples were collected from the six farms (12 samples per farm) to estimate levels of antibiotic residues. Results revealed that the prevalence of Escherichia coli in chicken thigh samples collected from Farms 4 and 5 were 25% (3/12) and 16.6% (2/12), respectively. In contrast, chicken breast samples from all farms were free from Escherichia coli. Serological identification of the five Escherichia coli isolates revealed that they were O91: H21 (EHEC) and O2: H6 (EPEC) with a prevalence of 16.7% (n=2) and 8.33% (n=), respectively in chicken thigh samples from Farm 4, while isolates from Farm 5 were serotyped as O78 (ETEC) and O44: H18 (EPEC) in chicken thigh samples with a prevalence rate of 8.33% for each serotype. Antibiotic resistance pattern using Vitek 2 revealed that all Escherichia coli isolates were ESBL negative and showed resistance to Trimethoprim/ Sulfamethoxazole, Piperacillin, Ticarcillin, and Ciprofloxacin but were sensitive to other antibiotics used. HPLC was used to estimate the level of antibiotic residues in chicken liver. No residues were detected in 2 farms in addition to the control farm. Liver samples obtained from the remaining 3 tested farms were found to contain Doxycycline (851 mg/kg), Oxytetracycline (115 mg/kg) and Oxytetracycline (117 mg/kg), respectively. This study underlines that more strict regulations are required for the use of antimicrobial drugs in poultry industry as well as the monitoring for drug residues prior to marketing.

Key words: Escherichia coli, antimicrobial resistance, Vitek 2, HPLC, drug residues


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed M. Elsenduony
Articles by Wafaa M. Bakr
Articles by Abd El Rahman M. Wafa
Articles by Eman A. Omran
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Elsenduony MM, Bakr WM, Wafa AERM, Omran EA. Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. AJVS. 2022; 74(1): 69-77. doi:10.5455/ajvs.55367


Web Style

Elsenduony MM, Bakr WM, Wafa AERM, Omran EA. Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. https://www.alexjvs.com/?mno=55367 [Access: July 30, 2022]. doi:10.5455/ajvs.55367


AMA (American Medical Association) Style

Elsenduony MM, Bakr WM, Wafa AERM, Omran EA. Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. AJVS. 2022; 74(1): 69-77. doi:10.5455/ajvs.55367



Vancouver/ICMJE Style

Elsenduony MM, Bakr WM, Wafa AERM, Omran EA. Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. AJVS. (2022), [cited July 30, 2022]; 74(1): 69-77. doi:10.5455/ajvs.55367



Harvard Style

Elsenduony, M. M., Bakr, . W. M., Wafa, . A. E. R. M. & Omran, . E. A. (2022) Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. AJVS, 74 (1), 69-77. doi:10.5455/ajvs.55367



Turabian Style

Elsenduony, Mohamed M., Wafaa M. Bakr, Abd El Rahman M. Wafa, and Eman A. Omran. 2022. Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. Alexandria Journal of Veterinary Sciences, 74 (1), 69-77. doi:10.5455/ajvs.55367



Chicago Style

Elsenduony, Mohamed M., Wafaa M. Bakr, Abd El Rahman M. Wafa, and Eman A. Omran. "Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver." Alexandria Journal of Veterinary Sciences 74 (2022), 69-77. doi:10.5455/ajvs.55367



MLA (The Modern Language Association) Style

Elsenduony, Mohamed M., Wafaa M. Bakr, Abd El Rahman M. Wafa, and Eman A. Omran. "Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver." Alexandria Journal of Veterinary Sciences 74.1 (2022), 69-77. Print. doi:10.5455/ajvs.55367



APA (American Psychological Association) Style

Elsenduony, M. M., Bakr, . W. M., Wafa, . A. E. R. M. & Omran, . E. A. (2022) Monitoring of Escherichia coli in Chicken Carcasses Collected from Different Poultry Farms and their Antimicrobial Sensitivity with Special Reference to Drug Residues in The Liver. Alexandria Journal of Veterinary Sciences, 74 (1), 69-77. doi:10.5455/ajvs.55367





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 16 times [Click to see citing articles]

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 12 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 9 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 9 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 7 times [Click to see citing articles]