Molecular studies on Clostridium perfringens  from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants

Ibrahim M. Aboreeda; Ahmed A. Bekheet; Sami A. Khalil

doi:10.5455/ajvs.86157

2025, Vol: 84

84Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Original Article

AJVS. 2021; 70(2): 7-14

doi: 10.5455/ajvs.86157

Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants

Ibrahim M. Aboreeda, Ahmed A. Bekheet, Sami A. Khalil.

Abstract
At the most cases, C. perfringens has the ability to form biofilms in order to persist in the environment as, the predominant structural state of bacteria in nature is biofilms which increase the bacterial tolerance to disinfection that can lead to economic and health concerns. Two hundred eighteen samples (88 liver samples and 130 intestinal samples) were collected from clinically affected chickens with necrotic enteritis and revealed the presence of C. perfringens with percentage of 94.6 % and 78.4% respectively with total percentage 86.5 %. Twenty suspected C. perfringens isolates were examined by PCR and the results revealed that all of the examined samples were identified as C. perfringens and all samples revealed bands of AppB gene at 109 bp, 17 of 20 samples gave iolD gene bands at 98 bp and 13 of 20 samples gave ripH gene bands at 105 bp. These isolates were used for antiobiotic susceptibility testing and results showed that all isolates were highly susceptible to imipenem. Also, Most of isolates were highly susceptible to Ampicillin and Amoxicillin but, all isolates were resistant to Sulphamethoxazole trimethoprim, Colistin, Tylosine and Lincomycin and also, all isolates showed multidrug resistance. And these isolates were screened for biofilm producing ability and results showed that all the twenty isolates were strong biofilm producers except three isolates which were moderate biofilm producers, Nine types of disinfectants commonly used on poultry farms were used to test their efficacy on C. perfringens biofilms and Sodium hypochlorite 0.027% was the most efficient disinfectant on C. perfringens biofilms.

Key words: Clostridium Perfringens, PCR, Alpha toxin, Biofilms, disinfectants.

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Ibrahim M. Aboreeda Articles by Ahmed A. Bekheet Articles by Sami A. Khalil
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Aboreeda IM, Bekheet AA, Khalil SA. Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants. AJVS. 2021; 70(2): 7-14. doi:10.5455/ajvs.86157

Web Style

Aboreeda IM, Bekheet AA, Khalil SA. Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants. https://www.alexjvs.com/?mno=86157 [Access: December 01, 2024]. doi:10.5455/ajvs.86157

AMA (American Medical Association) Style

Aboreeda IM, Bekheet AA, Khalil SA. Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants. AJVS. 2021; 70(2): 7-14. doi:10.5455/ajvs.86157

Vancouver/ICMJE Style

Harvard Style

Aboreeda, I. M., Bekheet, . A. A. & Khalil, . S. A. (2021) Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants. AJVS, 70 (2), 7-14. doi:10.5455/ajvs.86157

Turabian Style

Aboreeda, Ibrahim M., Ahmed A. Bekheet, and Sami A. Khalil. 2021. Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants. Alexandria Journal of Veterinary Sciences, 70 (2), 7-14. doi:10.5455/ajvs.86157

Chicago Style

Aboreeda, Ibrahim M., Ahmed A. Bekheet, and Sami A. Khalil. "Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants." Alexandria Journal of Veterinary Sciences 70 (2021), 7-14. doi:10.5455/ajvs.86157

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article