E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy.

Cited By: 9

Abstract
A total number of 170 fishes (100 fresh water, 40 brackish water, and 30 marine water fishes) from different farms in Alexandria, Kafr Elsheikh, and Behira governorates were collected from different water salinities. The prevalence of A.hydrophila was 47% (38% in fresh water fish, 65% brackish water fish, 53.3% in marine water fish). A.hydrophila isolates were tested for pathogenicity by inoculation into blood agar and skimmed milk agar 1% for hemolytic and proteolytic activity.
All isolates showed β hemolysis and proteolytic activity. A.hydrophila isolates were inoculated into trypticase soya broth with different NaCl concentration (0.5, 1, 2, 3, 4, 5, 6%) and turbidity was measured by photometer at wave length 610 nm. The higher NaCl concentration, the lower turbidity was found. Moreover, reduction % of total bacterial count of A.hydrophila in relation to different concentration of NaCl in TSB after 10 fold serial dilutions by surface plating technique was calculated.
Hemolysin and aerolysin genes were detected by PCR from the isolates from different sources. A.hydrophila isolates from fresh, brackish and marine fish showed high resistance to Ampicillin ï´¾M10ï´¿, Erythromycin ï´¾E15ï´¿ Nalidixic acid (NA30), and Spectinomycin (SPT10). On the other hand, A.hydrophila isolates from fresh, brackish and marine fish showed high sensitivity to Enrofloxacin (EF10), Ofloxacin ï´¾OFX5 ï´¿and Gentamicin (CN10ï´¿. Polymixin ï´¾PB300 u ï´¿showed activity against A.hydrophila from brackish and marine fishes only. Doxycycline ï´¾DO30ï´¿ and Nitrofurantoin ï´¾F300u) were highly effective against brackish water isolates than others for the field application. Isolation of A.hydrophila from different water salinities raises the public health concern and the importance to find suitable methods to control the infection.

Key words: A.hydrophila, Fresh, Brackish, Marine fishes, PCR


 
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REFERENCES
Ali, M.N. 1994. studies on bacterial and mycotic affections of freshwater fishes in aquaculture. Ph.D thesis, Fac. Vet. Mad. Cairo Univ., Egypt.
Austin, B., Austin, D. A. 2007. Bacterial fish pathogens: disease of farmed and wild fish. Springer Science & Business Media.
Baumann, P. A. U. L., Baumann, L. I. N. D. A. 1981. The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. The prokaryotes, 1, 1302-1331.
Cagatay, I.T., E.B. Þen, 2014. Detection of pathogenic Aeromonas hydrophila from rainbow trout (Oncorhynchus mykiss) farms in Turkey. Int. J. Agric. Biol. 16: 435‒438.
Cascón, A., Anguita, J., Hernanz, C., Sánchez, M., Fernandez, M., Naharro, G. 1996. Identification of Aeromonas hydrophila hybridization group 1 by PCR assays. Applied and environmental microbiology, 62(4), 1167-1170.
Chacon, M.R., Figuras, M.J. , Castro-Escarpulli, G.,. Soler, L., Guarro, J. 2003. Distrubution of virulence genes in clinical and environmental isolates of Aeromonas spp. Antonie V. Leewen, 84: 269‒278.
Chu, W. H., Lu, C. P. 2005. Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila. . Fish Dis.28(7): 437-441. [DOI via Crossref]    [Pubmed]   
Clinical and Laboratory Standards Institute (CLSI) (2012). Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement;[ provides updated tables for... M02-A11 and M07-A9]. National Committee for Clinical Laboratory Standards.
Hu, M., Wang, N., Pan, Z.H. ., Lu, C.P., Lui, Y.J. 2012. Identity and virulence properties of Aeromonas isolates from diseased fish, healthy controls and water environment in China. Lett. Appl. Microbiol., 55: 224‒233.
John, N. 2015. Distribution extracellular virulence factors and antibiogram of motile aeromonads in fresh water ornamental fishes and immune response of cyprinus carpio against aeromonas hydrophila infection.
Khan, A. A., Cerniglia, C. E. 1997. Rapid and sensitive method for the detection of Aeromonas caviae and Aeromonas trota by polymerase chain reaction. Letters in applied Mcrobio.24(4), 233-239.
Kingombe, C.I.B., J.Y. D' Aoust, G. Huys, L. Hofmann, M. Rao and J. Kwan, 2010. Multiplex PCR method for detection of three Aeromonas enterotoxin genes. Appl. Environ. Microbiol., 76: 425‒433
Kozaki, S., Kato, K., Aso, T., Kamata, Y., Sakaguchi, G. 1987. Activities of Aeromonas hydrophila hemolysin and their interaction with erythrocte membranes. Infec. Immun. 55: 1594‒1599.
Musa, N., L.S. Wei and W. Wee, 2008. Phenotypic and Genotypic Characteristics of Vibrio Harveyi Isolated from Black Tiger Shrimp (Penaeus Monodon). World Applied Sci. J. 3(6): 885-902.
World Health Organization (WHO), 1999. Removing obstacles for healthy development.WHO, Geneva. 1999.
Mary, P., Chihib, N.-E., Charafeddine, O., Defives, C., Hornez, J.P., 2002. Starvation Survival and Viable but Nonculturable States in Aeromonas hydrophila. Microbiol. Ecol. 43, 250–258.
Padan, E., Krulwich, T. A. 2000. Sodium stress (pp. 117-130). ASM Press: Washington, DC.
Paniagua, C., Rivero, O., Anguita, J., Naharro, G. 1990. Pathogenicity factors and virulence for rainbow trout of motile Aeromonas spp.isolated from a river. J. Clin. Microbiol., 28: 350‒355urk Univ. J. Vet. Sci. 1: 6‒10.
Pianetti, A., Manti, A., Boi, P., Citterio, B., Sabatini, L., Papa, S.. Bruscolini, F. 2008. Determination of viability of Aeromonas hydrophila in increasing concentrations of sodium chloride at different temperatures by flow cytometry and plate count technique. International journal of food microbial.,127(3): 252-260.
Saglam, Y.S., Iþik, N., Arslan, A., Erer, H. 2006. Isolation and pathologic research of Aeromonas hydrophila ve Yersinia ruckeri in Rainbow Trout (Oncorhynchus mykiss W.1792) in Erzurum Region. Ataturk Univ. J. Vet. Sci. 1: 6‒10.
Salama, A. N. N. I. K. A., Morgan, I. J., Wood, C. M. 1999. The linkage between Na+ uptake and ammonia excretion in rainbow trout: kinetic analysis, the effects of (NH4) 2SO4 and NH4HCO3 infusion and the influence of gill boundary layer pH. J. Experiment. Biol. 202(6): 697-709.
Sambrook, Joseph, Edward F. Fritsch, and Tom Maniatis. 1989. Molecular cloning. Vol. 2. New York: Cold spring harbor laboratory press.
Santos, Y., Toranza, A.E., Barja, J.L., Nieto, T.P. Villa, T.G. 1988. Virulence properties and enterotoxin production of Aeromonas strains isolated from fish. Infec. Immun. 56: 3285‒3293.
Takahashi E, Ozaki H, Fujii Y, Kobayashi H, Yamanaka H, et al. (2014) Properties of Hemolysin and Protease Produced by Aeromonas trota. PLoS ONE 9(3): e91149. [DOI via Crossref]   
Tanekhy, M., Kono, T., Sakai, M. 2009. Expression profile of cytokine genes in the common carp species Cyprinus carpio L. following infection with Aeromonas hydrophila. Bull. Eur. Ass. Fish Pathol. 29(6): 198.
Uma, A., Rebecca, G., Meena, S. , Saravanabava, K., 2010. PCR detection of putative aerolysin and hemolysin genes in an Aeromonas hydrophila isolate from infected Koi carp (Cyprinus carpio). Tamil. J. Vet. Anim. Sci., 6: 31‒33
Wang, G., C.G., Clark, C. Lui, C. Pucknell, C.K. Munro, T.M.A.C. Kruk, R. Caldeira, D.L. Woodward, F.G. Rodgers, 2003. Detection and chracterization of the hemolysin genes in Aeromonas hydrophila, Aeromonas sobria by multiplex PCR. J. Clin. Microbiol., 41: 1048‒1054
Watanabe, N., Morita, K., Furukawa, T., Manzoku, T., Endo, E., Kanamori, M. 2004. Sequence analysis of amplified DNA fragments containing the region encoding the putative lipase substrate-binding domain and genotyping of Aeromonas hydrophila. Appl. Environ. Microbiol. 70: 145‒151.
Wood, J. M., Bremer, E., Csonka, L. N., Kraemer, R., Poolman, B., van der Heide, T., Smith, L. T. 2001. Osmosensing and osmoregulatory compatible solute accumulation by bacteria. Comparative Biochemistry and Physiology Part A: Mol. Integrative Physiol.130(3): 437-460.
Xia, C., Ma, Z.H., Rahman, H., Wu, Z.G. . 2004. PCR cloning and identification of the β-haemolysin gene of Aeromonas hydrophila from freshwater fishes in China. Aquacul. 229: 45‒53.

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How to Cite this Article
Pubmed Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. 2016; 48(2): 46-53. doi:10.5455/ajvs.208107


Web Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. https://www.alexjvs.com/?mno=208107 [Access: February 25, 2023]. doi:10.5455/ajvs.208107


AMA (American Medical Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. 2016; 48(2): 46-53. doi:10.5455/ajvs.208107



Vancouver/ICMJE Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. (2016), [cited February 25, 2023]; 48(2): 46-53. doi:10.5455/ajvs.208107



Harvard Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy (2016) Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS, 48 (2), 46-53. doi:10.5455/ajvs.208107



Turabian Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. 2016. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. Alexandria Journal of Veterinary Sciences, 48 (2), 46-53. doi:10.5455/ajvs.208107



Chicago Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. "Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes." Alexandria Journal of Veterinary Sciences 48 (2016), 46-53. doi:10.5455/ajvs.208107



MLA (The Modern Language Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. "Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes." Alexandria Journal of Veterinary Sciences 48.2 (2016), 46-53. Print. doi:10.5455/ajvs.208107



APA (American Psychological Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy (2016) Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. Alexandria Journal of Veterinary Sciences, 48 (2), 46-53. doi:10.5455/ajvs.208107





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