E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir.


Abstract
In the present study, attempts were made to prepare fluorescein-conjugated polyclonal antibodies to be used for accurate routine diagnosis of NDV. Ten male boskat rabbits, ten male guinea pigs and two male goats were subcutaneously hyperimmunized with NDV (Lasota strain). The mean HI antibody titers raised against NDV in rabbits, guinea pigs and goats were 8, 7 and 8.5log2, respectively. The globulin contents of the prepared antisera were precipitated by ammonium sulphate and then conjugated with FITC. In order to evaluate the accuracy of the prepared conjugates in diagnostic purposes, direct FAT was applied for identification of NDV in impression smears prepared from various tissues of experimentally infected chickens using tenfold serial dilutions of the prepared conjugated antisera. Rabbits, guinea pig and goat conjugates showed clear positive reactions up to the dilution of 1/100000, 1/10000 and 1/1000000, respectively. The overall findings indicated that accurate and precise diagnosis of ND can be achieved by direct FAT using fluorescein-conjugated anti-NDV polyclonal antibodies.

Key words: Newcastle disease virus, Hyperimmune sera, Fluorescien-isothiocyanate.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Shrouk Mohamed
Articles by Samy A. Khalil
Articles by Mohamed H. Khodeir
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. AJVS. 2016; 51(2): 52-57. doi:10.5455/ajvs.219722

Web Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. https://www.alexjvs.com/?mno=219722 [Access: December 01, 2024]. doi:10.5455/ajvs.219722

AMA (American Medical Association) Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. AJVS. 2016; 51(2): 52-57. doi:10.5455/ajvs.219722


Vancouver/ICMJE Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. AJVS. (2016), [cited December 01, 2024]; 51(2): 52-57. doi:10.5455/ajvs.219722


Harvard Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir (2016) Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. AJVS, 51 (2), 52-57. doi:10.5455/ajvs.219722


Turabian Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. 2016. Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. Alexandria Journal of Veterinary Sciences, 51 (2), 52-57. doi:10.5455/ajvs.219722


Chicago Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. "Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen." Alexandria Journal of Veterinary Sciences 51 (2016), 52-57. doi:10.5455/ajvs.219722


MLA (The Modern Language Association) Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir. "Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen." Alexandria Journal of Veterinary Sciences 51.2 (2016), 52-57. Print. doi:10.5455/ajvs.219722


APA (American Psychological Association) Style

Shrouk Mohamed, Samy A. Khalil, Mohamed H. Khodeir (2016) Rapid Detection of Newcastle Disease Virus (NDV) Antigen Using Fluorescein Isothiocynate-Conjugated Immunoglobulins to Replace Identification by Detection and to Add Antigen. Alexandria Journal of Veterinary Sciences, 51 (2), 52-57. doi:10.5455/ajvs.219722




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use