E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 89
89Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus

Basma A. Elkady, Emad M. Al-ebshahy, Samy A. Khalil, Amani A. Saleh.

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    • Abstract
    The present study was intended to prepare and evaluate the applicability of fluorescein-conjugated anti-RHDV polyclonal antibodies for routine diagnosis and research purposes. A total of 22 animals (10 rabbits, 10 guinea pigs and 2 goats) were immunized subcutaneously with locally prepared RHDV inactivated vaccine containing 210 HAU per ml (rabbits and guinea pigs received 0.5 ml per dose, mean while goats received 1ml). Five doses with 7 days intervals were applied. The last dose was simultaneously injected with Montanide™ ISA 206 oil adjuvant. Five weeks post-immunization, RHDV-specific HI antibody titers were significantly increased and reached to the peak level with a mean titer of 7, 8 and 8.5 log2 in guinea pig, rabbit and goat sera, respectively. The globulin fraction of the prepared antisera was calculated, successfully precipitated with ammonium sulphate and then conjugated with FITC. The application of direct IF test on impression smears prepared from tissues of experimentally infected rabbits with RHDV isolate Giza-2006, revealed that positive reactions were obtained with guinea pig, rabbit and goat conjugates up to a dilution of 10-4, 10-5 and 10-6respectively. These findings clearly indicate that fluorescein-conjugated polyclonal antibodies developed in the present study can be used for accurate and precise diagnosis of RHDV.

    Key words: Rabbit haemorrhagic disease virus (RHDV), Polyclonal antibodies, immunofluorescence, conjugate


    		 
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    How to Cite this Article
    Pubmed Style

    Elkady BA, Al-ebshahy EM, Khalil SA, Saleh AA. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. 2016; 51(2): 46-51. doi:10.5455/ajvs.219728

    Web Style

    Elkady BA, Al-ebshahy EM, Khalil SA, Saleh AA. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. https://www.alexjvs.com/?mno=219728 [Access: June 24, 2026]. doi:10.5455/ajvs.219728

    AMA (American Medical Association) Style

    Elkady BA, Al-ebshahy EM, Khalil SA, Saleh AA. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. 2016; 51(2): 46-51. doi:10.5455/ajvs.219728


    Vancouver/ICMJE Style

    Elkady BA, Al-ebshahy EM, Khalil SA, Saleh AA. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. (2016), [cited June 24, 2026]; 51(2): 46-51. doi:10.5455/ajvs.219728


    Harvard Style

    Elkady, B. A., Al-ebshahy, . E. M., Khalil, . S. A. & Saleh, . A. A. (2016) Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS, 51 (2), 46-51. doi:10.5455/ajvs.219728


    Turabian Style

    Elkady, Basma A., Emad M. Al-ebshahy, Samy A. Khalil, and Amani A. Saleh. 2016. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 46-51. doi:10.5455/ajvs.219728


    Chicago Style

    Elkady, Basma A., Emad M. Al-ebshahy, Samy A. Khalil, and Amani A. Saleh. "Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus." Alexandria Journal of Veterinary Sciences 51 (2016), 46-51. doi:10.5455/ajvs.219728


    MLA (The Modern Language Association) Style

    Elkady, Basma A., Emad M. Al-ebshahy, Samy A. Khalil, and Amani A. Saleh. "Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus." Alexandria Journal of Veterinary Sciences 51.2 (2016), 46-51. Print. doi:10.5455/ajvs.219728


    APA (American Psychological Association) Style

    Elkady, B. A., Al-ebshahy, . E. M., Khalil, . S. A. & Saleh, . A. A. (2016) Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 46-51. doi:10.5455/ajvs.219728




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    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

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