E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article
Online Published: 27 May 2016
 


Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals

Ismail. I. Abo-ghanema, Madiha S. Ibrahim, Bahig. R. Nemettallah, Hanan A. Ghoneim.


Abstract
The present study was aimed to produce hyperimmune chicken egg against rabbit hemorrhagic disease virus (RHDV) as well as studying immunogenic characters of the extracted IgY using antigen-antibody reaction tests (agar gel immunodiffusion and ELISA). The study was carried out on ten white Hy-Line layers which received initial immunization of 300µl of RHDV vaccine subcutaneously followed by three booster doses at time intervals of 2, 5 and 8 weeks after initial immunization. Throughout the experiment (14 weeks), regular blood sampling was made every week with daily collection of egg till further purification and detection of the purity of the isolated IgY by SDS-PAGE. Also, determination of the specificity and titers of purified IgY in hens' sera and their purified egg yolk samples was carried out using agar gel immunodiffusion test and indirect ELISA. Results showed presence of considerable titers of isolated IgY against RHDV. Therefore, the current study recommends immunization of hens with RHDV for production of egg yolk antibodies against such pathogen.

Key words: Rabbit hemorrhagic disease virus, IgY, Indirect ELISA


 
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How to Cite this Article
Pubmed Style

Abo-ghanema II, Ibrahim MS, Nemettallah BR, Ghoneim HA. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. 2016; 49(2): 133-140. doi:10.5455/ajvs.224376

Web Style

Abo-ghanema II, Ibrahim MS, Nemettallah BR, Ghoneim HA. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. https://www.alexjvs.com/?mno=230412 [Access: March 26, 2025]. doi:10.5455/ajvs.224376

AMA (American Medical Association) Style

Abo-ghanema II, Ibrahim MS, Nemettallah BR, Ghoneim HA. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. 2016; 49(2): 133-140. doi:10.5455/ajvs.224376


Vancouver/ICMJE Style

Abo-ghanema II, Ibrahim MS, Nemettallah BR, Ghoneim HA. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. (2016), [cited March 26, 2025]; 49(2): 133-140. doi:10.5455/ajvs.224376


Harvard Style

Abo-ghanema, I. I., Ibrahim, . M. S., Nemettallah, . B. R. & Ghoneim, . H. A. (2016) Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS, 49 (2), 133-140. doi:10.5455/ajvs.224376


Turabian Style

Abo-ghanema, Ismail. I., Madiha S. Ibrahim, Bahig. R. Nemettallah, and Hanan A. Ghoneim. 2016. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. Alexandria Journal of Veterinary Sciences, 49 (2), 133-140. doi:10.5455/ajvs.224376


Chicago Style

Abo-ghanema, Ismail. I., Madiha S. Ibrahim, Bahig. R. Nemettallah, and Hanan A. Ghoneim. "Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals." Alexandria Journal of Veterinary Sciences 49 (2016), 133-140. doi:10.5455/ajvs.224376


MLA (The Modern Language Association) Style

Abo-ghanema, Ismail. I., Madiha S. Ibrahim, Bahig. R. Nemettallah, and Hanan A. Ghoneim. "Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals." Alexandria Journal of Veterinary Sciences 49.2 (2016), 133-140. Print. doi:10.5455/ajvs.224376


APA (American Psychological Association) Style

Abo-ghanema, I. I., Ibrahim, . M. S., Nemettallah, . B. R. & Ghoneim, . H. A. (2016) Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. Alexandria Journal of Veterinary Sciences, 49 (2), 133-140. doi:10.5455/ajvs.224376




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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