E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 27 May 2016
 


Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals

Ismail. I. Abo-Ghanema, Madiha S. Ibrahim, Bahig. R. Nemettallah, Hanan A. Ghoneim.


Abstract
The present study was aimed to produce hyperimmune chicken egg against rabbit hemorrhagic disease virus (RHDV) as well as studying immunogenic characters of the extracted IgY using antigen-antibody reaction tests (agar gel immunodiffusion and ELISA). The study was carried out on ten white Hy-Line layers which received initial immunization of 300µl of RHDV vaccine subcutaneously followed by three booster doses at time intervals of 2, 5 and 8 weeks after initial immunization. Throughout the experiment (14 weeks), regular blood sampling was made every week with daily collection of egg till further purification and detection of the purity of the isolated IgY by SDS-PAGE. Also, determination of the specificity and titers of purified IgY in hens' sera and their purified egg yolk samples was carried out using agar gel immunodiffusion test and indirect ELISA. Results showed presence of considerable titers of isolated IgY against RHDV. Therefore, the current study recommends immunization of hens with RHDV for production of egg yolk antibodies against such pathogen.

Key words: Rabbit hemorrhagic disease virus, IgY, Indirect ELISA


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ismail. I. Abo-Ghanema
Articles by Madiha S. Ibrahim
Articles by Bahig. R. Nemettallah
Articles by Hanan A. Ghoneim
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. 2016; 49(2): 133-140. doi:10.5455/ajvs.224376

Web Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. https://www.alexjvs.com/?mno=230412 [Access: September 12, 2024]. doi:10.5455/ajvs.224376

AMA (American Medical Association) Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. 2016; 49(2): 133-140. doi:10.5455/ajvs.224376


Vancouver/ICMJE Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS. (2016), [cited September 12, 2024]; 49(2): 133-140. doi:10.5455/ajvs.224376


Harvard Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim (2016) Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. AJVS, 49 (2), 133-140. doi:10.5455/ajvs.224376


Turabian Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. 2016. Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. Alexandria Journal of Veterinary Sciences, 49 (2), 133-140. doi:10.5455/ajvs.224376


Chicago Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. "Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals." Alexandria Journal of Veterinary Sciences 49 (2016), 133-140. doi:10.5455/ajvs.224376


MLA (The Modern Language Association) Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim. "Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals." Alexandria Journal of Veterinary Sciences 49.2 (2016), 133-140. Print. doi:10.5455/ajvs.224376


APA (American Psychological Association) Style

Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim (2016) Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals. Alexandria Journal of Veterinary Sciences, 49 (2), 133-140. doi:10.5455/ajvs.224376




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine, Alexandria University
    Edfina, Behaira, Egypt.
    PO.Box: 22758
    Telefax: +20 45 2963526
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use