E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article
Online Published: 27 May 2016
 


Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim.


Abstract
In the current study, we compared the efficiency of PCR detection to culture-dependent isolation of E. coli, as a model, from the liver and intestinal contents. Further, the incidence differences of E. coli isolation from the liver and intestine. Samples were collected from birds suffering from respiratory manifestation and/or diarrhea. Ninety E. coli isolates were recovered from 60 birds (52 intestinal and 38 liver samples) by bacteriological culture on selective broth and selective agar. PCR was performed using pho-A gene as a general marker for E. coli on DNA directly purified from the samples and assigned PCR (a). Negative PCR (a) samples were cultured on broth and another PCR was done, assigned PCR (b). Bacteriological isolation was more sensitive than PCR (a) indicating that inhibitors in the samples could have reduced or totally blocked the amplification capacity of PCR (a), which limited its diagnostic usefulness. PCR (b) was more sensitive than PCR (a) and more practical than bacteriological isolation. Detection of three virulence genes; iut-A, iss and tsh, showed genotypic variations of avian pathogenic E. coli (APEC) isolated from the liver and the intestine. In conclusion, an enrichment step results in more sensitive PCR than culture performed one. Further, general genes rather than virulence genes should be used as an indicator for E. coli detection in infected materials.

Key words: E. coli, PCR, pho-A, colibacillosis


 
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How to Cite this Article
Pubmed Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . AJVS. 2016; 49(2): 141-148. doi:10.5455/ajvs.219057

Web Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . https://www.alexjvs.com/?mno=230413 [Access: September 12, 2024]. doi:10.5455/ajvs.219057

AMA (American Medical Association) Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . AJVS. 2016; 49(2): 141-148. doi:10.5455/ajvs.219057


Vancouver/ICMJE Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . AJVS. (2016), [cited September 12, 2024]; 49(2): 141-148. doi:10.5455/ajvs.219057


Harvard Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim (2016) Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . AJVS, 49 (2), 141-148. doi:10.5455/ajvs.219057


Turabian Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. 2016. Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . Alexandria Journal of Veterinary Sciences, 49 (2), 141-148. doi:10.5455/ajvs.219057


Chicago Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. "Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea ." Alexandria Journal of Veterinary Sciences 49 (2016), 141-148. doi:10.5455/ajvs.219057


MLA (The Modern Language Association) Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim. "Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea ." Alexandria Journal of Veterinary Sciences 49.2 (2016), 141-148. Print. doi:10.5455/ajvs.219057


APA (American Psychological Association) Style

Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim (2016) Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea . Alexandria Journal of Veterinary Sciences, 49 (2), 141-148. doi:10.5455/ajvs.219057




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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