Abstract
This study was designed to evaluate the efficacy of 3.5 g Humic acid (HA), 3.5gesterifiedglucomannan (EGM) and 5gHydrated Sodium Calcium Aluminosilicate (HSCAS) to counteract the toxic effects of 1 ppm aflatoxin in the broiler chicken7-35days of age.Three hundred 7-day-old broiler chicks were randomly divided into five treatment groups of 4 replicates (each contained 15 chicks). While chicks in group 1 were fed a basal diet free of toxin (control), group 2 chicks were fed a basal diet contaminated with 1ppm AF, the other three groups 3, 4 and 5 were fed contaminatedbasal diet supplemented with 3.5 g HA, 3.5 g GEM and 5 g HSCAS, respectively. All groups were kept under observation from 7-35 days of age. Results obtained can be summarized as follows:Feeding contaminated diets significantly decreased final body weight, BW gains and feed intake. Feed conversion rate (FCR) was lowest in the group consuming the aflatoxin diet (P < 0.05). Further, contaminated diet caused significant decreases in serum total protein, albumin, globulin, total lipids, calcium and phosphorus. Compared with the control, the contaminated diet with 1ppm AF significantly increased the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid and creatinine. Also, there was impairment in liver and Serum antioxidant enzyme activities,antibodies titer against Newcastle Disease (ND) and aflatoxin B1 residual in the liver associated with aflatoxin toxicity. Increased relative weights of liver and decreased dressing % were observed in chicks fed the AF- diet.The results cleared that treatment of aflatoxicated birds with 3.5g HA, 3.5 g GEM and 5 g HSCAS induced protection from the development of signs and lesions with significant (p<0.05) improvement of performance when compared with un-treated control group. HA, GEM and HSCAS treatment induced significant (p<0.05) amelioration of the measured organs body weights ratio, humoral immune response to Newcastle Disease (ND) and biochemical parameters in aflatoxicated chickens. In conclusion, GEM also improved the economic traits of broilers but was less effective than HA and more effective than HSCAS.

Key words: aflatoxin, broiler, adsorbent compounds, detoxification