Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens

Ahmed Hussien; Elshaimaa Ismael; Basant M. Sobhi; Aya M. Yassin; Khaled Y. Farroh; Khaled N. Fahmy

doi:10.5455/ajvs.306362

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Original Article
Online Published: 20 Mar 2026

AJVS. 2026; 89(0): 195-208

doi: 10.5455/ajvs.306362

Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens

Ahmed Hussien, Elshaimaa Ismael, Basant M. Sobhi, Aya M. Yassin, Khaled Y. Farroh, Khaled N. Fahmy.

Abstract
Abstract
Background
The efficacy of replacing conventional dicalcium phosphate (DCP) with nano-hydroxyapatite (NHA), with or without phytase, in the diets of broiler chickens was investigated over a 35-day feeding period.
Methods
A total of 250 one-day-old Ross 308 broiler chicks (as hatch) were randomly allocated into four different dietary groups, each with five replicates (12 chicks/replicate). The control (CON) group was fed the basal diet only, whereas two treatment groups were fed diets containing 0.1% NHA (NHA 0.1%) or 0.2% NHA (NHA 0.2%) without DCP or phytase. A fourth group (Phy + NHA 0.1%) was fed 0.1% NHA combined with phytase 5000 FTU/g (0.01%), also without DCP. Growth performance, carcass traits, tibia mineralization, mineral excretion, and blood biochemical indices were evaluated.
Results
Dietary supplementation of 0.1% NHA with 0.01% phytase 5G showed significant improvements (p ≤ 0.05) in body weight, weight gain, feed conversion ratio (FCR), and European Production Efficiency Factor (EPEF) compared to other groups. Moreover, tibia Ca and P % were maintained (p > 0.05) as a ratio of 2:1 among all groups, indicating that NHA could improve Ca and P bioavailability in bone. However, Ca (p = 0.002) and P (p = 0.003) excretion were significantly reduced in all NHA-fed groups compared with the control. Serum calcium concentration increased (p = 0.009) in birds receiving NHA diets, while the combination of 0.1% NHA with 0.01% phytase 5G resulted in the greatest enhancement of serum phosphorus (p = 0.001) compared with the NHA-only groups.
Conclusion
Conclusively, replacing DCP with 0.1% NHA, particularly when supplemented with phytase, enhanced growth performance, improved feed efficiency, and increased P utilization while preserving bone mineralization in broiler chickens. These findings support NHA as a promising alternative mineral source in poultry nutrition.

Key words: Nano-hydroxyapatite, Phytase, Tibia, Phosphorus excretion, Serum calcium

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How to Cite this Article

Pubmed Style

Hussien A, Ismael E, Sobhi BM, Yassin AM, Farroh KY, Fahmy KN. Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. AJVS. 2026; 89(0): 195-208. doi:10.5455/ajvs.306362

Web Style

Hussien A, Ismael E, Sobhi BM, Yassin AM, Farroh KY, Fahmy KN. Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. https://www.alexjvs.com/?mno=306362 [Access: April 16, 2026]. doi:10.5455/ajvs.306362

AMA (American Medical Association) Style

Hussien A, Ismael E, Sobhi BM, Yassin AM, Farroh KY, Fahmy KN. Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. AJVS. 2026; 89(0): 195-208. doi:10.5455/ajvs.306362

Vancouver/ICMJE Style

Hussien A, Ismael E, Sobhi BM, Yassin AM, Farroh KY, Fahmy KN. Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. AJVS. (2026), [cited April 16, 2026]; 89(0): 195-208. doi:10.5455/ajvs.306362

Harvard Style

Hussien, A., Ismael, . E., Sobhi, . B. M., Yassin, . A. M., Farroh, . K. Y. & Fahmy, . K. N. (2026) Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. AJVS, 89 (0), 195-208. doi:10.5455/ajvs.306362

Turabian Style

Hussien, Ahmed, Elshaimaa Ismael, Basant M. Sobhi, Aya M. Yassin, Khaled Y. Farroh, and Khaled N. Fahmy. 2026. Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens. Alexandria Journal of Veterinary Sciences, 89 (0), 195-208. doi:10.5455/ajvs.306362

Chicago Style

Hussien, Ahmed, Elshaimaa Ismael, Basant M. Sobhi, Aya M. Yassin, Khaled Y. Farroh, and Khaled N. Fahmy. "Evaluation of Nano-Hydroxyapatite as an Alternative to Inorganic Phosphorus Source, With and Without Phytase Supplementation, on the Productive Performance of Broiler Chickens." Alexandria Journal of Veterinary Sciences 89 (2026), 195-208. doi:10.5455/ajvs.306362

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