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Original Article
Online Published: 14 May 2022
 


Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources

Mahmoud Elafify, Basma Badawy, Mohamed Sayed-Ahmed, Radwa Shata, Marwa EL-Toukhy, Mayada Gwida.


Abstract
This study was conducted to evaluate the potential existence of pathogenicEscherichia coli in smallholding dairy buffaloes, their products as well as in some environmental sources. In total, 150 samples including an equal sample size (n = 25) of raw milk, Kareish cheese, water, feed, udder and rectal swabs were examined. Phenotypic and genotypic procedures were used to characterize the bacterial population. Twenty-eight isolates were confirmed as E. coli from the examined samples (18.66%). The recovered isolates were categorized as Enterohemorrhagic E. coli (n=11),Enteropathogenic E. coli(n=9), Enterotoxigenic E. coli(n= 5)and Enteroinvasive E. coli (n=3). All isolates were resistant to erythromycin followed by oxacillin (89.28%), and nalidixic acid (67.85%), while 96% of the recovered strains displayed high sensitivity to imipenem. A great proportion of the recovered E. coli (67.9%) exhibited multidrug resistance. The majority of isolates (92.9%) harbored stx1either alone or in association with stx2 which present in 60.7% (17/28) while eaeA genes were detected in 50% among the recovered isolates in association with stx1 or combined with stx2 (n =11). Twelve isolates (42.86%) expressed all the examined beta lactamase encoding genes; while only 25% of the isolates harbored all tested beta lactamase genes in common with stx1, stx2 and eaeA genes.The results herein indicated that the pathogenicE. coli could be present in the examined samples which pose a relevant threat and negative impact on animal as well as consumer health. The study emphasizes the necessity of developing long-term strategies to assure dairy food safety.

Key words: Pathogenic E. coli, Smallholding buffaloes ,Environment, Dairy products.


 
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How to Cite this Article
Pubmed Style

Elafify M, Badawy B, Sayed-Ahmed M, Shata R, EL-Toukhy M, Gwida M. Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. AJVS. 2022; 74(1): 31-40. doi:10.5455/ajvs.7695

Web Style

Elafify M, Badawy B, Sayed-Ahmed M, Shata R, EL-Toukhy M, Gwida M. Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. https://www.alexjvs.com/?mno=7695 [Access: September 12, 2024]. doi:10.5455/ajvs.7695

AMA (American Medical Association) Style

Elafify M, Badawy B, Sayed-Ahmed M, Shata R, EL-Toukhy M, Gwida M. Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. AJVS. 2022; 74(1): 31-40. doi:10.5455/ajvs.7695


Vancouver/ICMJE Style

Elafify M, Badawy B, Sayed-Ahmed M, Shata R, EL-Toukhy M, Gwida M. Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. AJVS. (2022), [cited September 12, 2024]; 74(1): 31-40. doi:10.5455/ajvs.7695


Harvard Style

Elafify, M., Badawy, . B., Sayed-Ahmed, . M., Shata, . R., EL-Toukhy, . M. & Gwida, . M. (2022) Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. AJVS, 74 (1), 31-40. doi:10.5455/ajvs.7695


Turabian Style

Elafify, Mahmoud, Basma Badawy, Mohamed Sayed-Ahmed, Radwa Shata, Marwa EL-Toukhy, and Mayada Gwida. 2022. Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. Alexandria Journal of Veterinary Sciences, 74 (1), 31-40. doi:10.5455/ajvs.7695


Chicago Style

Elafify, Mahmoud, Basma Badawy, Mohamed Sayed-Ahmed, Radwa Shata, Marwa EL-Toukhy, and Mayada Gwida. "Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources." Alexandria Journal of Veterinary Sciences 74 (2022), 31-40. doi:10.5455/ajvs.7695


MLA (The Modern Language Association) Style

Elafify, Mahmoud, Basma Badawy, Mohamed Sayed-Ahmed, Radwa Shata, Marwa EL-Toukhy, and Mayada Gwida. "Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources." Alexandria Journal of Veterinary Sciences 74.1 (2022), 31-40. Print. doi:10.5455/ajvs.7695


APA (American Psychological Association) Style

Elafify, M., Badawy, . B., Sayed-Ahmed, . M., Shata, . R., EL-Toukhy, . M. & Gwida, . M. (2022) Genetic Characterization of Pathogenic Escherichia Coli Isolated from Smallholding Dairy Buffaloes, their Products, and Some Environmental Sources. Alexandria Journal of Veterinary Sciences, 74 (1), 31-40. doi:10.5455/ajvs.7695




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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