Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks

Mona A. AbdelRahman; Karim Selim; Amany Adel

doi:10.5455/ajvs.24347

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Original Article
Online Published: 15 Jun 2022

AJVS. 2022; 74(1): 24-30

doi: 10.5455/ajvs.24347

Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks

Mona A. Abdelrahman, Karim Selim, Amany Adel.

Abstract
Poultry health problems of chickens are usually accompanied by bacterial systemic infections causing severe losses in flocks, sick birds were systemically could be infected by several bacteria regardless of the clinical signs. 16s RNA sequence is a modern approach for the diagnosis of bacterial infection, especially of multiple causative agents. Ten field cases suffering from systemic infections have been examined for bacterial causes, using conventional isolation and 16S rRNA sequencing, The ten sequenced samples were compatible with the results of isolation; six samples were similar to E.coli species with identity% = 97-99%, while it had similarity% < 96% with Salmonella enterica. While the other samples showed variable identity ranging from 94-99% with Salmonella enterica, E. coli, Enterobacter spp and Klebsiella spp
In conclusion, although conventional isolation is the golden standard method for bacterial diagnosis, however, the 16s RNA sequencing has elaborated excellence in diagnosis and identification of bacterial infection, concerning the time consuming, detection of multi-caused infections and sensitivity.

Key words: Keywords: 16sRNA, sequencing, poultry.

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Pubmed Style

Abdelrahman MA, Selim K, Adel A. Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks. AJVS. 2022; 74(1): 24-30. doi:10.5455/ajvs.24347

Web Style

Abdelrahman MA, Selim K, Adel A. Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks. https://www.alexjvs.com/?mno=24347 [Access: May 04, 2025]. doi:10.5455/ajvs.24347

AMA (American Medical Association) Style

Abdelrahman MA, Selim K, Adel A. Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks. AJVS. 2022; 74(1): 24-30. doi:10.5455/ajvs.24347

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Harvard Style

Abdelrahman, M. A., Selim, . K. & Adel, . A. (2022) Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks. AJVS, 74 (1), 24-30. doi:10.5455/ajvs.24347

Turabian Style

Abdelrahman, Mona A., Karim Selim, and Amany Adel. 2022. Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks. Alexandria Journal of Veterinary Sciences, 74 (1), 24-30. doi:10.5455/ajvs.24347

Chicago Style

Abdelrahman, Mona A., Karim Selim, and Amany Adel. "Comparative Study Between the Sequencing of 16sRNA gene and Traditional Identification Methods of Some Bacterial Pathogens Associated with A Systemic Bacterial Infections from Diseased Poultry Flocks." Alexandria Journal of Veterinary Sciences 74 (2022), 24-30. doi:10.5455/ajvs.24347

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APA (American Psychological Association) Style

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